A NEW HUMAN INTERFERON-γ MUTANT HARBORING EGF RECEPTOR-INTERFERING SEQUENCE POSSESSES HIGH ANTIPROLIFERATIVE ACTIVITY  

作　　者：王晓鸣 李玉英 金奇 张智清 侯云德 

机构地区：[1]National Laboratory of Molecular Virology and Genetic Engineering, Institute of Virology, Chinese Academy of Preventive Medicine, Beijing 100052, PRC

出　　处：《Science China Chemistry》1992年第1期84-91,共8页中国科学（化学英文版）

基　　金：Work supported by China National Expert Committee for Biotechnology Development

摘　　要：An interferon gamma (IFN-γ) mutant gene harboring an additionamal epidermal growth factor (EGF) sequence at its 3’ terminal was constructed and highly expressed in E. coli by using pBV220 as a vector. The new fusion protein-lFN-γ-EGF possesses not only high antiviral activity, but also high antiproliferative effect as demonstrated by ~3H-TdR incorporation method using CN Ⅱ ceils which are rich in EGF receptors.An interferon gamma (IFN-γ) mutant gene harboring an additionamal epidermal growth factor (EGF) sequence at its 3' terminal was constructed and highly expressed in E. coli by using pBV220 as a vector. The new fusion protein-lFN-γ-EGF possesses not only high antiviral activity, but also high antiproliferative effect as demonstrated by ~3H-TdR incorporation method using CN Ⅱ ceils which are rich in EGF receptors.

关 键 词：INTERFERON GAMMA EPIDERMAL growth factor protein engineering. 

分 类 号：O6[理学—化学]