STUDY ON WAYS OF FORCING INTERCELLULAR CYTOPLASM AND CHROMATIN MIGRATION TO TRANSFER EXTERNAL GENETIC SUBSTANCES TO PLANT CELLS BY ARTIFICIAL MOTIVE FORCE  

作　　者：吴伯骥 陈毅平 崔亚亚 郑国锠 杨庆兰 

机构地区：[1]Chengdu Institute of Biology Academia Sinica, Chengdu 610015, PRC [2]Cytology Lab of Lanzhou University, Lanzhou 730000, PRC

出　　处：《Science China Chemistry》1992年第3期291-303,共13页中国科学（化学英文版）

基　　金：This work is supported by the National "863" Advanced Technique Development Project in China and the "7.5" Science and Technique Development Project in China

摘　　要：A new cell engineering technique (L. B. technique) was established in our Lab. At first, the physical and chemical methods were used to facilitate the reestablishment of intercellular contacts and plasmodesma channels between different parents, thus forcing cytoplasm and chromatin to pass the cell wall with different qualities and quantities from one cell to others, through the enlarged intercellular plasmodesma channels or the vulnerable regions and the holes on the cell wall formed differently in growth and thickness in the process of cell wall formation to introduce external genetic substances or gene groups into plant cells. There are different ways, frequencies and strengths for the migration between the cells in different growth and development regions or the same growth and development region. In this paper we advance the mechanism of cytoplasm and chromatin migration through the cell wall: There are a large number of plasmodesma channels or vulnerable regions and holes different in growth and thickness during the formation of the cell walls between meristematic cells. Low osmotic pressure and low pH value K+ solution treatments force the augmentation of plasmic streaming, and the streaming of karyolymph, the relaxation of the cell wall, the formation of more holes on the cell wall and the enlargement of plasmodesma channels in meristematic cells. All these make a proper structural and physiological condition for migrating through cell walls. Osmotic pressure and centrifugal forces are the practical forces forcing cytoplasm and chromatin to migrate extensively and transfer external genetic substances or gene groups to plant cells.A new cell engineering technique (L. B. technique) was established in our Lab. At first, the physical and chemical methods were used to facilitate the reestablishment of intercellular contacts and plasmodesma channels between different parents, thus forcing cytoplasm and chromatin to pass the cell wall with different qualities and quantities from one cell to others, through the enlarged intercellular plasmodesma channels or the vulnerable regions and the holes on the cell wall formed differently in growth and thickness in the process of cell wall formation to introduce external genetic substances or gene groups into plant cells. There are different ways, frequencies and strengths for the migration between the cells in different growth and development regions or the same growth and development region. In this paper we advance the mechanism of cytoplasm and chromatin migration through the cell wall: There are a large number of plasmodesma channels or vulnerable regions and holes different in growth and

关 键 词：meristematic cell LOW OSMOTIC pressure and LOW pH value K+ solution L. B. technique INTERCELLULAR PLASMODESMA channel. 

分 类 号：O6[理学—化学]