细胞癌基因在人脑胚胎发育过程中的表达  

The Expression of Oncogenes in the Development of Human Brain

作　　者：孙宁[1] 孙芝琳[1] 

机构地区：[1]华西医科大学生物化学与分子生物学研究所重组DNA室

出　　处：《华西医科大学学报》1992年第1期9-12,共4页Journal of West China University of Medical Sciences

基　　金：国家自然科学基金

摘　　要：作者用分子杂交技术观察了正常4～7月龄胎儿脑的各个部位和正常人脑胚胎发育过程中癌基因的表达。结果显示:多数细胞癌基因的表达水平在小脑和枕叶较高,在颞叶的表达水平较低;人脑组织胚胎发育过程中,c-myc、L-myc、N-myc基因的表达情况类似;erbB基因在胚胎期脑中均有较强的表达,而在正常成人脑中未见其表达;Ha-ras、c-fos基因在人脑胚胎发育的中后期表达水平较高;mos基因在新生儿脑中表达最强。研究结果提示:这7种癌基因可能在人脑的正常胚胎发育过程中起重要作用。Seven oncogenes (c-myc, L-myc, N-myc, v-erbB, c-fos, Ha-ras and mos)were used as the probe to detect the totalRNA of every part (cerebellum, temporalfolium, frontal folium and occipital folium)in human brain from 2 cases of 6 mon fe-tus and the total RNA in fetal development(4 mon, 5 mon, 6 mon, 7 mon, and newborn)of human brain tissue by RNA dot hybri-dization analysis. The results showed thatmost oncogenes were expressed at high levelin cerebellum and occipital folium of 6 monfetus and at low level in temporal folium;there was similar expression of c-myc, L-myc and N-myc in fetal development ofhuman brain tissue, and the highest levelwas in the 5 mon fetal brain. There was noexpression of erbB gene in normal human brain. The high level expression of Ha-rasand c-fos genes was in the middle and laterstages of fetal development. These resultssuggested that c-myc, L-myc,N-myc,erbB,Ha-ras, c-fos and mos genes might play animportant role in the fetal development ofhuman brain.

关 键 词：细胞癌基因 人脑胚胎发育 基因表达 斑点杂交 

分 类 号：R[医药卫生]