RECOGNITION SEQUENCE SPECIFICITY OF SIGNAL PEPTIDASE I AND THE ROLE OF SIGNAL PEPTIDE IN SECRETION OF PROTEIN IN Bacillus subtilis  

作　　者：周毅 蒋如璋 

机构地区：[1]Laboratory for Biotechnology, Department of Biology, Nonkai University

出　　处：《Science China Chemistry》1991年第9期1082-1091,共10页中国科学（化学英文版）

基　　金：Basic research of "863" High Technology Program, supported by the National Natural Science Foundation of China.

摘　　要：By recombinant DNA technology, the N-terminal of the β-protein encoding region of plasmid pUBHO is fused with the structure gene of α-amylase from Bacillus licheniformis. This gene fusion is called βAmy. It is able to transcribe and translate in phase. Protein fusion can be secreted into the medium mediated by β-signal peptide. The efficiency of secretion is about 10% of the synthesized pre-α-amylase. By comparing the secretion capacities and analysing the restriction sites on β-Amy genes and the molecular weights of the mature α-amylase secreted by B. subtilis harbouring different plasmids, it is indicated in vivo that the recognition and cleavage sequence for signal peptidase I of B. subtilis is Ala-Ala-Ala Ala. The results also indicate that the secretion of the α-amylase in B. subtilis is in accordance with the post-translational transportation mode.By recombinant DNA technology, the N-terminal of the β-protein encoding region of plasmid pUBHO is fused with the structure gene of α-amylase from Bacillus licheniformis. This gene fusion is called βAmy. It is able to transcribe and translate in phase. Protein fusion can be secreted into the medium mediated by β-signal peptide. The efficiency of secretion is about 10% of the synthesized pre-α-amylase. By comparing the secretion capacities and analysing the restriction sites on β-Amy genes and the molecular weights of the mature α-amylase secreted by B. subtilis harbouring different plasmids, it is indicated in vivo that the recognition and cleavage sequence for signal peptidase I of B. subtilis is Ala-Ala-Ala Ala. The results also indicate that the secretion of the α-amylase in B. subtilis is in accordance with the post-translational transportation mode.

关 键 词：Α-AMYLASE β-Amy gene β-signal peptide SIGNAL peptid-ase Ⅰ POST-TRANSLATIONAL transfer Bacillus subtilis. 

分 类 号：O6[理学—化学]