无菌兔、无菌豚鼠的培育及生物学特性与应用  

Studies on Raising Gerrafree Rabbits and Guinea Pigs and their Biological Characteristics and Applications

作　　者：王荫槐[1] 景绍亮[1] 王安京[1] 周尚珍 孙淑华[1] 刘素梅[1] 赵铭[1] 

机构地区：[1]中国医学科学院实验动物研究所

出　　处：《中国比较医学杂志》1991年第1期27-30,共4页Chinese Journal of Comparative Medicine

摘　　要：选用新西兰种孕兔和短毛种孕豚鼠,经剖腹产获无菌仔畜。饲养于YDG隔离器内,前期喂以人工乳,离乳后喂以固体饲料。使用的全部器材和饲料均经严格灭菌,以保证动物的无菌状态.在无菌兔和无菌豚鼠培育成功的基础上对其生物学特性及应用进行了研究。研究结果表明:采用人工饲养方法和无菌状态的保持技术是成熟的。两种动物生长发育良好,并呈现多见于无菌动物的一些生物学特征,如盲肠异常增大,免疫系统不发达等.两种动物可用于宏生生物与微生生物;微生生物与微生生物之间相互依存竞争关系的研究.可用于制备血清抗体以及建立三级SPF动物和二级清洁级动物种群.Germfree rabbits and guinea pigs were delivered by hysterotomy in New Zealand pregnant rabbits and short-haired albino guinea pigs, the entire uterus was removed into the isolator before the foetuses were dissected. During the early weeks of the life artificial milk was fed and followed by solid diets. All the materials and diets were sterilizated thoroughly for the maintenance of germfree condition. Based on the successful raising of the germfree animals,investigations on the biological characteristics and applications have been done. It has been found that the procedures in hand-feeding and germ free maintenance were successful. Germfree animals grow in good condition with the significant characteristics which was seen in the most other germfree animals, i.e. enlarged cecum, undeveloped immune system, etc. By using defined germfree animal models more researches can be done,such as studies on the relationships between macro-orgnisms and microorgnisms, relationships between micro-orgnisms. the producing of antibodies and the establishments of SPF and cleaning laboratory animal stocks.

关 键 词：无菌兔 无菌豚鼠 隔离器 

分 类 号：R[医药卫生]