香烟烟雾凝聚物对人胎肾类上皮细胞转化作用的研究  

Transformation of Human Fetal Renal Epithelia—like Cells in Vitro Induced by Cigarette Smoke Condensate

作　　者：陈家堃[1] 吴中亮[1] 陈慧虹 冯健威 高宏光[1] 杜应秀[1] 

机构地区：[1]广州医学院化学致癌研究所

出　　处：《广州医学院学报》1991年第3期6-10,共5页Academic Journal of Guangzhou Medical College

摘　　要：用浓度为10和15μg/ml的香烟烟雾凝聚物处理人胚肾原代细胞24小时,一个月左右细胞开始发生转化。转化细胞的超微结构出现一些特征性的改变,细胞的染色体数目变化明显,DNA合成旺盛,细胞在半固体琼脂上生长,目前体外传代培养已在4年以上,即已获得不死性。In this paper renal epithelial like cells from human fetal were used for the cell transformation studies. The primary cultured cells were treated with the solvent extract of cigarette smoke condensate at concentrations of 10 and 15μg/ml for 24hrs. About 30-40 days after treatment some characteristic changes in the treated cells were observed. Morphological features of the treated cells under light and electron microscopy were similar to that of malignant cells. The chromosomes of these cells changed drastically in number. The rate of incorporation of H-TdR into DNA was higher in the treated cells than in the control cells. Treated cells grew on agar plates and showed an increased lifespan. Immortalization of treated cells was also induced. These results indicate that transformation of human fetal renal epithelial like cells can be induced by cigarette smoke and that this system may be useful for cell transformation studies.

关 键 词：香烟烟雾凝聚物 人胚肾细胞 细胞转化 

分 类 号：R[医药卫生]