Preliminary study of immunodiagnosis of trichinosis by ELISA using urea-soluble antigen of Trichinella spiralis  

作　　者：张津利 赖福春 

机构地区：[1]Department of Parasitology,Third Military Medical College,Chongqing 630038

出　　处：《Journal of Medical Colleges of PLA(China)》1991年第1期84-87,92,共5页中国人民解放军军医大学学报（英文版）

摘　　要：Urea-soluble antigens of Trichinella spiralis infected larva were preparedand the efficiency of the antigens in diagnosis of trichinosis by ELISA wasstudied.Specific IgG antibodies were detected by ELISA using urea-solubleantigens in 91.67% (22/24) of 24 serum samples taken from patients withtrichinosis and 91.67% (11/12) of 12 blood spots on filter paper.Specific IgMantibodies were seen in 95.24% (20/21) of 21 serum samples from patients withtrichinosis using the antigens.Sera of patients infected with Schistosoma japonicumhad some cross teactions to the antigens,but the sera of mice infected withSchistosoma japoni(?) Ancylostoma caninum larva and Plasmodium yoelti were allnegative in the test.Urea-soluble antigens of Trichinella spiralis infected larva were prepared and the efficiency of the antigens in diagnosis of trichinosis by ELISA was studied.Specific IgG antibodies were detected by ELISA using urea-soluble antigens in 91.67% (22/24) of 24 serum samples taken from patients with trichinosis and 91.67% (11/12) of 12 blood spots on filter paper.Specific IgM antibodies were seen in 95.24% (20/21) of 21 serum samples from patients with trichinosis using the antigens.Sera of patients infected with Schistosoma japonicum had some cross teactions to the antigens,but the sera of mice infected with Schistosoma japoni(?) Ancylostoma caninum larva and Plasmodium yoelti were all negative in the test.

关 键 词：TRICHINOSIS FLISA ANTIGENS TRICHINELLA spiralis 

分 类 号：R[医药卫生]