Separation and identification of the major components of Photocarcinorin and their tumor-photobiological activities  

作　　者：陈文晖 段祥生 许德余 

机构地区：[1]Division of Medicinal Chemistry,Laboratory for Antimalarial Drug Research,Second Military Medical University,Shanghai 200433

出　　处：《Journal of Medical Colleges of PLA(China)》1991年第4期309-318,共10页中国人民解放军军医大学学报（英文版）

摘　　要：It has been shown by the results-of HPLC analysis in combination with spectrographicdeterminations that PsD-007 is composed of 7 different porphyrins,In order of the proportion inPsD-007,they are:3 （or 8）-（l-methoxyethyl）-8 （or 3）-（l-hydroxyethyl）-deuteroporphyrin Ⅸ（MHD）;3,8-di-（l-methoxyethyl）-deuteroporphyrin Ⅸ（DMD）;3（or 8）-（l-methoxyethyl）-8 （or3）-vinyl-deuteroporphyrin Ⅸ（MVD）;3（or 8）-（l-hydroxyethyl）-8（or 3）-vinyl-deuteroporphyrin Ⅸ（HVD）;hernatoporphyrin Ⅸ （Hp）;protoporphyrin Ⅸ （Pp） and 3（or 8）-（O-aceylethyl）-8（or 3）-（l-hydroxyethyl）-deuteroporphrin Ⅸ （AHD）,which presented only in crude PsD-007 and hasbeen transformed into MHD and Hp,respectively during the separation and preparing the clinicalpreparation of PsD-007.Structures of these porphyrins were further eonfirmed by the corre-sponding anthentic samples obtained by synthetic method.It was found on the basis of the experi-mental data of photosensitizing ability in cell-free systems and photoinactivation of human cancercells in vitro as well as efficacy of photodynamic therapy for sarcoma.S₁₈₀ in mice of the ma-jor components MILD,DMD and MVD composed of which more over 85% of the totalamount of PsD-007,that they all exhibited comparatively high photosensitizing ability andphotodynamic effects on cancer cells and tram-planted animal tumor.It has been shown by the results-of HPLC analysis in combination with spectrographic determinations that PsD-007 is composed of 7 different porphyrins,In order of the proportion in PsD-007,they are:3 (or 8)-(l-methoxyethyl)-8 (or 3)-(l-hydroxyethyl)-deuteroporphyrin Ⅸ (MHD);3,8-di-(l-methoxyethyl)-deuteroporphyrin Ⅸ(DMD);3(or 8)-(l-methoxyethyl)-8 (or 3)-vinyl-deuteroporphyrin Ⅸ(MVD);3(or 8)-(l-hydroxyethyl)-8(or 3)-vinyl-deuteroporphyrin Ⅸ (HVD);hernatoporphyrin Ⅸ (Hp);protoporphyrin Ⅸ (Pp) and 3(or 8)-(O-aceylethyl)-8(or 3)- (l-hydroxyethyl)-deuteroporphrin Ⅸ (AHD),which presented only in crude PsD-007 and has been transformed into MHD and Hp,respectively during the separation and preparing the clinical preparation of PsD-007.Structures of these porphyrins were further eonfirmed by the corre- sponding anthentic samples obtained by synthetic method.It was found on the basis of the experi- mental data of photosensitizing ability in cell-free systems and photoinactivation of human cancer cells in vitro as well as efficacy of photodynamic therapy for sarcoma.S_(180) in mice of the ma- jor components MILD,DMD and MVD composed of which more over 85% of the total amount of PsD-007,that they all exhibited comparatively high photosensitizing ability and photodynamic effects on cancer cells and tram-planted animal tumor.

关 键 词：PHOTOCARCINORIN NEOPLASMS photodynamic therapy photolocalization photochemodiagnosis and PHOTOCHEMOTHERAPY photosensitizing ability PHOTOINACTIVATION photobiological activity SEPARATION and identificatio 

分 类 号：R[医药卫生]