病毒性心肌炎患者血请СохВ病毒抗体测定  

Detection of Coxsackie-B-Virus-Specific antibody in patients with viral myocarditis

作　　者：李芳秋 杨毓华 徐敬臻[2] 李露言 

机构地区：[1]全军医学检验中心微生物科 [2]解放军第91医院检验科 [3]全军医学检验中心心脏科

出　　处：《医学研究生学报》1991年第3期201-202,283-284,共4页Journal of Medical Postgraduates

摘　　要：建立了一个可测定 CoxB 1～6型病毒抗体的 ELISA 法,测定了40例病毒性心肌炎、31例其它心脏病及257例健康人的血清。抗 CBV 的阳性率分别为72.5%、16.1%和8.2%,心肌炎患者抗 CBV 阳性率及抗体滴度均显著高于两对照组,说明 ELISA 法测定 CBV抗体有助于病毒性心肌炎的病原学诊断。Coxsackie B(Ⅰ-Ⅵ)viruses are the major agents of myocar- ditis,but it is lacking aetiological evidence in the diagnosis.We develop- ed a simple,sensitive and time-saving ELISA method to detect anti-CBV -IgG so as to provide the evidence of CBV infection for patients with myocarditis. Sera from 40 patients with myocarditis were tested and the results showed that the percentage with specific antibody is 72.5%,much higher than those of control(8.2%)of the healthy and control(16.1%)with other heart disease.Six sera showed of anti-CBV positive in ELISA had the ac- tivity of neutralizing Cox B viruses on Hela cells.Extensive heterotypic antibody responses were observed.

关 键 词：病毒性心肌炎 CoxB 病毒抗体 酶联免疫吸附试验 

分 类 号：R[医药卫生]