茶树人工诱变技术的研究 Ⅳ.茶树辐照损伤的生理机制  

Study on the Techniques of Mutagensis in Tea Plant Ⅳ.Mechanism of Radiation Damage in Tea Plant

作　　者：杨跃华[1] 林树祺 

机构地区：[1]中国农业科学院茶叶研究所

出　　处：《茶叶科学》1991年第1期19-24,共6页Journal of Tea Science

基　　金：农01-04课题

摘　　要：对茶树辐照后自由基和抗坏血酸含量的动态变化及细胞膜抗性的研究结果表明,随着辐照剂量的增加,茶树体内的自由基含量呈线性增加,叶片中超氧物歧化酶活性下降,叶片的相对电导率增大,细胞膜脂质过氧化加剧,抗坏血酸含量降低而抗坏血酸酶活性增大,茶树的生理损伤加大。因而认为,茶树辐照后,体内自由基浓度的累积,诱发了超氧物歧化酶活性下降,自由基的产生与清除机制的失调,进一步加剧了细胞膜脂质过氧化,导致了细胞膜的损伤,是造成茶树辐照损伤的重要原因。The changes of the free radical concentration,the ascorbic acid contentand the membrane resistance of radiated tea plant were determined.Resultsshowed that,with the increasing of the radiative dose,the free radicalconcentration in tea plant increased significantly,SOD activity in the leafdecreased,the permeability and lipid peroxidation of the membrane inten-sified,the ascorbic acid content in the leaf declined but the activity ofascorbic acid oxidase increased,resulting in heavier damade to the radiatedtea plant.The radiation damage was caused by the accumulation of thefree radical resulting in the decline of SOD activity,disorder of the freeradical producing-eliminating mechanism,intensity of the membrane lipidperoxidation and the damage of cell membrane.

关 键 词：茶树 电离辐照 人工诱变 生理损伤 损伤机制 

分 类 号：S571.1[农业科学—茶叶生产加工]