锌指转录因子Snail在喉癌中的表达及其与不同亚群喉癌细胞侵袭和转移的相关性研究  

Expression of Snail in laryngeal carcinoma and its relationship with invasion and metastasis in subgroup laryngeal carcinoma cells

作　　者：于丹[1] 刘岩[1] 赵雪[1] 程金章[1] 齐欣萌[1] 金春顺[1] 

机构地区：[1]吉林大学第二临床医院耳鼻咽喉头颈外科,吉林长春130041

出　　处：《中国生化药物杂志》2014年第1期1-4,共4页Chinese Journal of Biochemical Pharmaceutics

基　　金：吉林省自然科学基金项目(20130101151JC);教育部博士点新教师基金项目(20120061120092)

摘　　要：目的探讨锌指转录因子Snail在喉鳞状细胞癌中的表达情况及其与临床特征的关系,通过检测不同喉癌细胞亚群中Snail的表达研究其与喉癌侵袭转移的相关性。方法免疫组化法检测吉林大学第二医院耳鼻咽喉头颈外科48例喉癌组织和20例癌旁对照组织手术标本Snail蛋白的表达情况,并分析Snail与临床特征的相关性。采用RT—PCR及Western blot和细胞免疫化学染色检测CD 44^+CD 133^+、CD 44^-和CD 133^-不同亚群喉癌细胞中Snail和粘附分子E—cadherin的表达情况,并与侵袭及转移进行相关性探讨。结果 Snail在喉癌组织中表达水平显著高于癌旁对照组织(P<0.01)。Snail的表达与喉癌的临床分期、淋巴结转移、肿瘤分化程度密切相关(P<0.05),而与患者的性别、年龄、临床分型等无关。RT—PCR及Western blot检测结果均证实,Snail在CD 44^+CD 133^+喉癌细胞中的表达水平明显高于CD 44^-和CD 1333^-喉癌细胞,而粘附分子E—cadherin在CD 44^+CD 133^+喉癌细胞中的表达水平明显低于CD 44^-和CD 133^-喉癌细胞,细胞免疫组化染色证实CD 44^+CD 133^+喉癌亚群细胞中Snail和E—cadherin的表达呈负相关。结论 Snail的表达与喉癌的侵袭转移密切相关,且在高致瘤亚群CD 44^+CD 133^+喉癌细胞中呈显著高表达,与粘附分子E—cadherin的表达呈负相关,与喉癌细胞的侵袭和转移具有密切相关性。Objective To study the expression of Snail in laryngeal carcinoma and to explore its relationship with invasion and metastasis in subgroup laryngeal carcinoma cells. Method The expression of Snail protein was examined by immunohistochemistry staining in the tissue of laryngeal carcinoma. The statistical evaluation was performed to detect the correlation between the Snail protein expression and clinical features. In different subgroups of laryngeal carcinoma cells (CD 44+CD 133+cells, CD 44 and CD 133-cells), the expression of Snail and adhesion molecules E-cadherin were detected by RT-PCR,Western blot test and immunochemical staining which were studied in the correlation with invasion and metastasis. Results The result of immunohistochemical staining revealed that Snail was moderately or highly expressed in the tissue of laryngeal carcinoma significantly and higher than those in adjacent non-cancerous tissues(P<0.01). Expression of Snail was highly correlated with lymph node metastasis, tumor differentiation and clinical classification(P<0.05).However, it was not related to the age , gender and clinical type. RT-PCR and Western blot test results confirmed that the expression level of Snail was significantly higher in CD 44+CD 133+laryngeal cancer cells than in CD 44-and CD 133-laryngeal cancer cells, otherwise the expression level of E-cadherin in CD 44+CD 133+laryngeal cancer cells was significantly lower than in the CD 44-and CD 133-laryngeal cancer cells. Cell immunohistochemical staining confirmed the expression of Snail and E-cadherin were negatively correlated in CD 44+CD 133+laryngeal cancer cells. Conclusion Over expression of Snail in laryngeal carcinoma is closely related to the development of laryngeal cancer and lymph node metastasis. The expression of Snail in the CD 44+CD 133+laryngeal cancer cell subgroup is negatively correlated with adhesion molecules E-cadherin, which is a close correlation with invasion and metastasis of laryngeal cancer cells.

关 键 词：喉癌 SNAIL E-CADHERIN 肿瘤转移 上皮细胞-间质转化 

分 类 号：R739.91[医药卫生—肿瘤]