蒽贝素联合紫杉醇抑制肺癌A549细胞的增殖和荷瘤裸鼠肿瘤的生长研究  

Inhibitation of embelin and paclitaxel on lung cancer A549 cell proliferation and tumor growing in tumor-bearing nude mice

作　　者：张春辉[1] 孙平[1] 

机构地区：[1]华中农业大学医院,湖北武汉430070

出　　处：《中国生化药物杂志》2014年第2期7-9,共3页Chinese Journal of Biochemical Pharmaceutics

基　　金：国家自然科学基金(81171365)

摘　　要：目的研究蒽贝素联合紫杉醇对A549细胞的增殖抑制作用和对肺癌异位瘤的生长抑制作用。方法采用MTT法研究蒽贝素联合紫杉醇对A549细胞的增值抑制作用。流式细胞术检测蒽贝素联合紫杉醇诱导A549细胞的凋亡率。构建裸鼠A549肺癌异位瘤模型,研究蒽贝素联合紫杉醇对肺癌异位瘤的生长抑制作用。结果蒽贝素联合紫杉醇对A549细胞的增殖抑制作用显著强于蒽贝素组和紫杉醇组,差异具有统计学意义(P<0.01)。细胞凋亡实验结果显示,蒽贝素联合紫杉醇诱导A549细胞凋亡率分别是蒽贝素组和紫杉醇组的4.8倍和2.4倍,差异具有统计学意义(P<0.01)。荷瘤裸鼠治疗实验结果显示,蒽贝素联合紫杉醇对肺癌的生长抑制作用显著强于其他组(P<0.01)。结论蒽贝素能够联合紫杉醇有效抑制A549细胞的增殖及荷瘤裸鼠肿瘤的生长,是一种潜在的肺癌治疗方法。Objective To evaluate the effect of embelin combined with paclitaxel on the inhibition of A 549 lung cancer cell proliferation and tumor growing in tumor bearing nude mice. Methods The anti-proliferation efficiency of embelin combined with paclitaxel were evaluated by MTT assay, and A 549 cell apoptosis were evaluated by lfow cytometry. A 549 cells were xenografted in mice to establish the animal model, which were used to evaluate the effect of anti-tumor. Results compared to saline group、embelin group and paclitaxel group, the (paclitaxel+embelin) group could inhibit the growth of A 549 cells effectively(P<0.05). The embelin combined with paclitaxel induced the apoptosis of A 549 cells more effective than paclitaxel alone. The (paclitaxel+embelin) group significantly inhibited the growth of tumor tissue. Conclusion the paclitaxel can inhibit the growth of A 549 cells, the embelin can induce the apoptosis of A 549 cells, and the combination of paclitaxel and embelin may be a potentially effective treatment for lung cancer.

关 键 词：蒽贝素 紫杉醇 肺癌 

分 类 号：R734.2[医药卫生—肿瘤]