补中益气汤含药血清对A549/DDP细胞生长周期作用的影响  

Effect of Buzhong Yiqi Decoction serum on cell cycle of A 549/DDP cell

作　　者：井欢[1] 唐莹[2] 于丹[1] 刘春英[1] 

机构地区：[1]辽宁中医药大学基础医学院病理教研室,辽宁沈阳110032 [2]辽宁中医药大学基础医学院人体解剖教研室,辽宁沈阳110032

出　　处：《中国生化药物杂志》2014年第2期48-50,共3页Chinese Journal of Biochemical Pharmaceutics

基　　金：辽宁省自然基金资助项目(20102145)

摘　　要：目的评估补中益气汤含药血清对A549/DDP细胞生长周期的影响效果。方法体外培养人肺腺癌A549/DDP细胞,实验分组为:空白对照组,补中益气汤组和脾细胞处理组。用不同浓度的补中益气汤含药血清和脾细胞刺激A549/DDP细胞,采用MTT法(Methylthiazolyldiphenyl-tetrazolium bromide,MTT)测定2种方法对细胞的直接杀伤作用;流式细胞术检测2种方法对细胞凋亡的影响;荧光显微镜观察2种方法对凋亡小体产生的影响。结果MTT法显示同空白对照组相比,补中益气汤含药血清对细胞生长均有抑制作用(P<0.05),且该法同脾细胞作用后效果类似;2种方法处理后,流式细胞术均可见特征性的细胞凋亡峰;在荧光显微镜下可见凋亡细胞。结论补中益气汤含药血清在体外能阻滞A549/DDP细胞的生长增殖,诱导该细胞发生凋亡,且效果同脾细胞类似。Objective To study the effect of Buzhong Yiqi Decoction serum on cell cycle of A549/DDP cell. Methods A549/DDP cells and splenocytes cells were cultured in vitro and divided randomly into three groups:control group, Buzhong Yiqi Decoction group and splenocyte group. A 549/DDP cells were stimulated by different concentrations of Buzhong Yiqi Decoction serum and spleen cells, the direct killing effect on cells were measured by MTT method, cell apoptosis were detected by lfow cytometric analysis and the apoptotic body were observed by immunolfuorescence method. Results MTT results showed that cell growth were inhibited by Buzhong Yiqi Decoction serum in contrast with control groups, and the effect was same as spleen cells, and both treated groups had similar effects. Marked apoptotic peak and the apoptotic body were seen by lfow cytometric analysis and lfuorescence microscope in both two groups. Conclusion Buzhong Yiqi Decoction could inhibit the proliferation of A549/DDP cells in vitro and induced cell apoptosis, which is the similar as spleen cells.

关 键 词：补中益气汤 A 549/DDP细胞 细胞周期 细胞凋亡 

分 类 号：R734.2[医药卫生—肿瘤]