喜泊分联合放疗对小鼠H22肝癌移植瘤的抑瘤增敏作用研究  

Inhibitation and radiosensitization effects of hematoporphyrin and radiotherapy on H22 liver cancer mice

作　　者：刘伟[1,2] 徐鑫[3] 刘媛媛[4] 刘斌[1] 王喜梅[2] 

机构地区：[1]河北省人民医院肿瘤科,河北石家庄050000 [2]河北中医学院护理学院,河北石家庄050000 [3]河北省人民医院急诊科,河北石家庄050000 [4]河北医科大学研究生学院,河北石家庄050000

出　　处：《中国生化药物杂志》2014年第3期57-60,共4页Chinese Journal of Biochemical Pharmaceutics

摘　　要：目的观察光敏剂喜泊分对小鼠H22肝癌移植瘤的抑瘤增敏效果。方法建立H22肝癌细胞小鼠移植瘤模型,将荷瘤小鼠随机分为对照组、单纯放疗组、小剂量喜泊分加放疗组(联合Ⅰ组)、中剂量喜泊分加放疗组(联合Ⅱ组)、大剂量喜泊分加放疗组(联合Ⅲ组)共5组,观察第比较5组小鼠14天瘤体积、抑瘤率、增敏q值、小鼠生存时间、病理、细胞凋亡等指标。结果喜泊分联合放疗能够抑制小鼠移植瘤生长;各联合治疗组q值均>1,均有放疗增敏效果,以联合治疗Ⅲ组均值最大;联合组较其他组细胞凋亡率升高,但组间比较差异无统计学意义;联合治疗组较对照组小鼠生存时间延长,以联合Ⅱ组最长,放疗组最短。结论喜泊分联合放疗对小鼠H22肝癌具有抑瘤增敏效果,且在一定范围内(5 mg/kg^20 mg/kg)其作用与药物浓度呈正相关性;喜泊分联合放疗治疗小鼠H22肝癌可延长小鼠生存期;其增敏机制可能与诱导细胞凋亡有关。Objective To observe the inhibitation and radiosensitization effect of hematoporphyrin and radiotherapy on H22 liver cancer mice.Methods Mice models of liver cancer were established by subcutaneous injection of H22 cells,tumor-bearing mice were randomly divided into:control group, simple radiotherapy group, small dose hematoporphyrin with radiotherapy group (combined treatment group Ⅰ), middle dose hematoporphyrin with radiotherapy group(combined treatment group Ⅱ),and high dose hematoporphyrin with radiotherapy group (combined treatment group Ⅲ).Tumor volumes,inhibition rate,sensitizing factor,the survival time of mice,pathology,apoptosis and other indicators in five groups were observed after14 days. Results Hematoporphyrin and radiotherapy can inhibit tumor growth in mice,The q values in three combined treatment groups were all above 1.0 at different time points ,and combined treatment groupⅢwas the highest.The three combined treatment groups had higher apoptosis rate than control group and simple radiotherapy group,but the difference within groups were not statistically significant. The survival time in three combined treatment groups were higher than control group,the combined treatment group Ⅱ was the longest and simple radiotherapy group was the shortest.Conclusion Hematoporphyrin has tumor inhibitition and radiation sensitizing effect on H22 liver cancer mice,and the effect was positive correlation with drug dose. Hematoporphyrin combined with radiotherapy can extend the lifespan of mice in H22 liver cancer,its sensitizing mechanism may be association with induction of H22 cell apoptosis.

关 键 词：肝癌 喜泊分 抑瘤率 放射增敏 凋亡 

分 类 号：R735.7[医药卫生—肿瘤]