蒽贝素联合阿霉素对乳腺癌细胞MCF-7增殖和荷瘤裸鼠肿瘤生长的影响  

Effect of embelin and doxorubicin on the proliferation of MCF-7 cell and growth of MCF-7 bearing nude mice

作　　者：李宗祥[1] 孙平[1] 

机构地区：[1]华中农业大学医院,湖北武汉430070

出　　处：《中国生化药物杂志》2014年第4期4-7,11,共4页Chinese Journal of Biochemical Pharmaceutics

基　　金：国家自然科学基金(81171365)

摘　　要：目的探讨阿霉素(doxorubicin,DOX)联合X连锁凋亡抑制蛋白小分子抑制剂蒽贝素(embelin,EB)对乳腺癌细胞MCF-7增殖及荷瘤裸鼠肿瘤生长的影响。方法根据加入药物的不同将MCF-7细胞分为生理盐水组、阿霉素干预组、蒽贝素干预组和联合干预组(蒽贝素+阿霉素)。MTT法检测各组对乳腺癌MCF-7细胞增殖的影响。流式细胞术检测各组肿瘤细胞凋亡情况。构建乳腺癌MCF-7细胞肿瘤球模型,探讨阿霉素联合蒽贝素对肿瘤球的生长抑制作用。建立乳腺癌裸鼠移植瘤模型,探讨阿霉素联合蒽贝素对乳腺癌异位瘤的生长抑制作用。结果乳腺癌MCF-7细胞在给药后48h,联合干预组对肿瘤细胞的增殖抑制率显著大于其他给药组,差异具有统计学意义(P<0.01);细胞凋亡实验和肿瘤球生长抑制实验都表明联合干预组的肿瘤细胞抑制能力显著强于其他组,差异具有统计学意义(P<0.01);荷瘤小鼠实验结果显示,联合干预组的肿瘤生长抑制作用显著强于其他组,差异具有统计学意义(P<0.01)。结论阿霉素与蒽贝素均具有抑制乳腺癌增殖的作用,2者联合应用能够发挥协同作用,增强抗肿瘤能力。Objective To explore the effect of X-linked inhibitor of apoptosis protein(XIAP)combined with doxorubicin on the proliferation of MCF-7 cell and growth of MCF-7 bearing nude mice.Methods The anti-proliferation efficiency of doxorubicin and embelin were determined by MTT assay.MCF-7 cell apoptosis induced by embelin and doxorubicin were detected by Flow cytometry.Anti-tumor ability of embelin and doxorubicin were evalued with tumor spheroids test.MCF-7 cell were xenografted to mice to establish the animal model,which was used to evaluate the effect of anti-tumor. Results Compared with saline group,embelin group and doxorubicin group,the combination(doxorubicin +embelin)group inhibited the growth of MCF-7 cells effectively(P<0.01).The combination group induced the apoptosis of MCF-7 cells more effectively than doxorubicin alone(P<0.01), and significantly inhibit the growth of tumor in vitro and in vivo than other groups(P<0.01).Conclusion The combination of doxorubicin and embelin may be used as a potentially effective treatment method for breast cancer.

关 键 词：阿霉素 蒽贝素 乳腺癌 

分 类 号：R737.9[医药卫生—肿瘤]