Expression analysis of reactive oxygen species detoxifying enzyme genes in Anopheles stephensi during Plasmodium berghei midgut invasion  

作　　者：RK Chaitanya P Sridevi K Surendra Kumar Babu S Mastan K Arun Kumar A Dutta-Gupta 

机构地区：[1]School of Life Sciences,University of Hyderabad

出　　处：《Asian Pacific Journal of Tropical Medicine》2014年第9期680-684,共5页亚太热带医药杂志（英文版）

摘　　要：Objective:To investigate the involvement of reactive oxygen species detoxification system in Anopheles stephensi during Plasmodium berghei midgut invasion.Methods:Eight key reactive oxygen species metabolizing enzymes were cloned and characterized,and their expression was monitored in parasite-infected mosquitoes.Results:Superoxide anion detoxifying superoxide dismutases(Fe/Mn SOD,Cu/Zn SOD 2,Cu/Zn SOD 3A,and Cu/Zn SOD 3B)depicted varied expression patterns.Fe/Mn SOD expression declined,whereas Cu/Zn SOD expression was elevated in the infected mosquitoes.Peroxidases,catalase and glutathione peroxidase showed lack of induction in expression during the Plasmodium berghei infection.Further,expression of thioredoxin reductase increased in the infected mosquitoes,whereas gluthathione S-transferase levels decreased markedly.Conclusions:Detoxification enzymes may play a role in modulating host immunity and parasite transmission.Objective:To investigate the involvement of reactive oxygen species detoxification system in Anopheles stephensi during Plasmodium berghei midgut invasion.Methods:Eight key reactive oxygen species metabolizing enzymes were cloned and characterized,and their expression was monitored in parasite-infected mosquitoes.Results:Superoxide anion detoxifying superoxide dismutases(Fe/Mn SOD,Cu/Zn SOD 2,Cu/Zn SOD 3A,and Cu/Zn SOD 3B)depicted varied expression patterns.Fe/Mn SOD expression declined,whereas Cu/Zn SOD expression was elevated in the infected mosquitoes.Peroxidases,catalase and glutathione peroxidase showed lack of induction in expression during the Plasmodium berghei infection.Further,expression of thioredoxin reductase increased in the infected mosquitoes,whereas gluthathione S-transferase levels decreased markedly.Conclusions:Detoxification enzymes may play a role in modulating host immunity and parasite transmission.

关 键 词：Anophelesstephensi Detoxificationenzymes GENEEXPRESSION MALARIA OXIDATIVESTRESS Plasmodiumberghei Reactiveoxygenspecies 

分 类 号：R382.31[医药卫生—医学寄生虫学]