~1H nuclear magnetic resonance-based metabolomics reveals sex-specific metabolic changes of gastrodin intervention in rats  

作　　者：Xin Li Yuan-Wei Jia Jun-Song Wang Ming-Hua Yang Kelvin D.G.Wang Ling-Yi Kong 

机构地区：[1]State Key Laboratory of Natural Medicines,Department of Natural Medicinal Chemistry,China Pharmaceutical University [2]Anhui provincial Center for Drug Clinical Evaluation,Yijishan Hospital,Wannan Medical College [3]Center for Molecular,Metabolism,School of Environmental and Biological Engineering,Nanjing University of Science and Technology

出　　处：《Asian Pacific Journal of Tropical Medicine》2014年第10期811-818,共8页亚太热带医药杂志（英文版）

基　　金：funded by the National Science Foundation of China(81173526);the Fundamental Research Funds for the Central Universities(No.30920130112014);the Program for New Century Excellent Talents in University,State Education Ministry of China(#NCET-11-0738);the Program for Changjiang Scholars and Innovative Research Team in University(PCSIRT-IRT1193);the Priority Academic Program Development of Jiangsu Higher Education Institutions(PAPD)

摘　　要：Objective:To explore^1H nuclear magnetic resonance-based metabolomics on sex-specific metabolic changes of gastrodin intervention in rats.Methods:In this research,~1H NMR-based metabolomics was used for the first time to investigate metabolic changes following chronic intervention with gastrodin in rats.Results:24 endogenous metabolites were identified.Body weight.daily diet and the total volume of urine in in each day of each rat were measured synchronously.Modifications in 12 metabolites were observsd following gastrodin intervention,indicating gastrodin-induced alterations in carbohydrate and energy metabolism.Interestingly,these metabolic changes were not totally identical in female and male rats.Some metabolic changes arising from gastrodin intervention showed sexual dimorphism including LDL/VLDL and lactate which were on the decrease in the female but on the increase in the male,together with arginine/ornithine,creatine,and glycerol which were on the increase in the female but on the decrease in the male.While the decrease in pyruvate,succinate and glutamate was only shown in the male and the increase in valine,α-ketoglutarate and glucose was only in the female.Conclusions:This resesrch shows the sex-specific metabolic response to GAS intervention,weather GAS is a healthy dietary supplement for the male merits furtherObjective:To explore^1H nuclear magnetic resonance-based metabolomics on sex-specific metabolic changes of gastrodin intervention in rats.Methods:In this research,~1H NMR-based metabolomics was used for the first time to investigate metabolic changes following chronic intervention with gastrodin in rats.Results:24 endogenous metabolites were identified.Body weight.daily diet and the total volume of urine in in each day of each rat were measured synchronously.Modifications in 12 metabolites were observsd following gastrodin intervention,indicating gastrodin-induced alterations in carbohydrate and energy metabolism.Interestingly,these metabolic changes were not totally identical in female and male rats.Some metabolic changes arising from gastrodin intervention showed sexual dimorphism including LDL/VLDL and lactate which were on the decrease in the female but on the increase in the male,together with arginine/ornithine,creatine,and glycerol which were on the increase in the female but on the decrease in the male.While the decrease in pyruvate,succinate and glutamate was only shown in the male and the increase in valine,α-ketoglutarate and glucose was only in the female.Conclusions:This resesrch shows the sex-specific metabolic response to GAS intervention,weather GAS is a healthy dietary supplement for the male merits further investigation

关 键 词：GASTRODIN Metabolomics ~1H NMR spectroscopy SEX DIFFERENCES Health protection 

分 类 号：R285.5[医药卫生—中药学]