Impact of antibacterial drugs on human serum paraoxonase-1(hPON1)activity:an in vitro study  

作　　者：Hakan Syt Elif Duygu Kaya Skr Beydemir 

机构地区：[1]Department of Primary Education,Faculty of Education,Bayburt University,69000 Bayburt,Turkey [2]Department of Food Engineering,Faculty of Engineering,Igdir University,76000 lgdir,Turkey [3]Department of Chemistry,Faculty of Science,Atatrk University,25240 Erzurum,Turkey

出　　处：《Asian Pacific Journal of Tropical Biomedicine》2014年第8期603-609,共7页亚太热带生物医学杂志（英文版）

基　　金：Supported by Atatrk University Scientific Research Project Fund(BAP-2009/81)

摘　　要：Objective:To investigate the in vitro effects of the antihacterial drugs,mcropenem trihydrate.piperacillin sodium,and cefoperazone sodium,on the activity of human serum paraoxonase mPOND.Methods:hPQN1 was purified from human serum using simple chromatographic methods.including DEAE-Sephadex anion exchange and Sephadex G-200 gel filtration chromatography.Results:The three antihacterial drugs decreased in vitro hPON1 activity.Inhibition mechanisms meropcnem trihydrate was noncompetitive while piperacillin sodium and cefoperazone sodium were competitive.Conclusions:Our results showed that antihacterial drugs significantly inhibit hPON1 activity,both in vitro,with rank order meropenem trihydrate piperacillin sodium cefoperazone sodium in vitro.Objective:To investigate the in vitro effects of the antihacterial drugs,mcropenem trihydrate.piperacillin sodium,and cefoperazone sodium,on the activity of human serum paraoxonase mPOND.Methods:hPQN1 was purified from human serum using simple chromatographic methods.including DEAE-Sephadex anion exchange and Sephadex G-200 gel filtration chromatography.Results:The three antihacterial drugs decreased in vitro hPON1 activity.Inhibition mechanisms meropcnem trihydrate was noncompetitive while piperacillin sodium and cefoperazone sodium were competitive.Conclusions:Our results showed that antihacterial drugs significantly inhibit hPON1 activity,both in vitro,with rank order meropenem trihydrate piperacillin sodium cefoperazone sodium in vitro.

关 键 词：PARAOXONASE Inhibition MEROPENEM trihydrate PIPERACILLIN SODIUM CEFOPERAZONE SODIUM 

分 类 号：R969[医药卫生—药理学]