SPIO标记的c-erbB2反义探针用于活体荷瘤裸鼠MR的最佳扫描时间探讨  

Optimal Time for MRI Scanning of Tumors in BALB/c Mice Using c-erbB2 Antisense Probe Labeled with Superparamagnetic Iron Oxide Nanoparticles

作　　者：温志鹏[1] 刘海燕[1] 文明[1] 贺海容[1] 谭书德[1] 李少林[1] 

机构地区：[1]重庆医科大学附属第一医院放射科,重庆400016

出　　处：《四川大学学报（医学版）》2013年第5期708-712,共5页Journal of Sichuan University(Medical Sciences)

基　　金：国家自然科学基金(No.30940021);重庆市自然科学基金(CSTC No.2008BB5209);重庆市卫生局医学科研基金(No.062025)资助

摘　　要：目的探讨超顺磁性氧化铁(SPIO)标记的c-erbB2反义寡脱氧核苷酸探针用于活体荷瘤裸鼠MR的最佳扫描时间。方法荷瘤裸鼠30只,注射反义探针(12.0mg Fe/kg)并于不同时间点(注射前,注射后60min、180min、360min、720min及1 440min)同步行MRI扫描。在完成一个时间点扫描后,立即处死荷瘤裸鼠5只,取出肿瘤组织,行HE及普鲁士蓝染色,光学显微镜下观察;测量肿瘤组织及邻近肌肉组织各时间点信号强度。结果成功建立荷瘤裸鼠模型,成瘤率100%。对比荷瘤裸鼠在反义探针注射前后的不同时间点扫描的信号强度,发现肿瘤组织在注射后360min信号变化最明显,在MR扫描图像上能清楚显示。HE染色发现肿瘤组织结构紊乱,存在大量异性肿瘤细胞,排列呈"癌巢"状,在各时间点表现相似;而普鲁士蓝染色发现各观察时间点标本的肿瘤组织中均散在分布斑点状蓝色铁颗粒,以注射反义探针后360min分布最密集。结论 SPIO标记的c-erbB2反义寡脱氧核苷酸探针能靶向于肿瘤组织,用于BALB/c裸鼠MR的最佳扫描时间为注射后360min。Objective To determine the optimal time for MRI scanning of tumors in BALB/c mice using c-erbB2 antisense probe labeled with superparamagnetic iron oxide nanoparticles.Methods SK-Br-3 tumor-bearing BALB/c mice(n=30)were injected with antisense probe(12.0mg Fe/kg).MRI scanning was performed on 5mice before the injection and 60 min,180 min,360 min,720 min and 1 440 min after the injection,respectively.Tumor tissues were taken immediately after the scanning and fixed with 10% formalin and paraffin-embedded sections.The MRI signal strength of the tumors and adjacent muscles were compared with changes detected under a microscope using HE and Prussian blue staining.Results SK-Br-3 tumors were introduced to the BALB/c mice successfully.The strongest signal intensity was detected by the MRI 360 min after injection with the antisense probe.The pathological examination revealed structural disorders of the tumor issues,with a large number of special-shaped cells arranged in a cancer nest shape.Punctuate blue iron particles were observed in all of the tumor issues,with the greatest density occurring at 360min after the injection with the antisense probe.Conclusion The optimal time for MRI scanning of tumors in BALB/c mice using c-erbB2 antisense probe labeled with superparamagnetic iron oxide nanoparticles should be set at 360min after injection.

关 键 词：超顺磁氧化铁 反义探针 活体动物 MR成像 扫描时间 

分 类 号：R73-36[医药卫生—肿瘤]