基于染色体单片段代换系的水稻糙米率QTL的定位  

QTL Mapping of Brown Rice Rate Using Chromosome Single Segment Substituted Lines in Rice

作　　者：周勇[1] 高云[1] 朱松松[1] 朱金燕[2] 王军[2] 裔传灯[1] 仲维功[2] 梁国华[1] 

机构地区：[1]教育部植物功能基因组学重点实验室,江苏省作物遗传生理重点实验室,扬州大学,江苏扬州225009 [2]国家水稻改良中心南京分中心,江苏省农业科学院粮食作物研究所,江苏南京210014

出　　处：《华北农学报》2013年第6期1-5,共5页Acta Agriculturae Boreali-Sinica

基　　金：国家重点基础研究发展计划项目(2010CB125904);江苏省科技支撑计划项目(BE2012309);国家与江苏省粮食丰产科技工程项目(No.2011BAD16B03/BE2009425);江苏省农业科技自主创新基金项目(CX(12)1003-2);江苏省植物功能基因组学重点实验室开放课题(K12003)

摘　　要：以广陆矮4号为受体,日本晴为供体的119个染色体单片段代换系为材料,通过单因素方差分析和Dunnett’s多重比较,分析染色体单片段代换系与受体亲本之间糙米率的差异,对代换片段上的糙米率相关QTL进行鉴定。以P≤0.001为阈值共检测到2个糙米率QTL,利用代换作图法将其分别定位在第6染色体和第9染色体上,2个QTL均表现为增效作用,增效位点来自于粳稻亲本日本晴,其加性效应值分别为1.49%和1.32%,加性效应百分率分别为1.89%和1.68%。该研究对进一步发掘和利用新的水稻糙米率QTL具有重要意义,同时对提高水稻糙米率、增加稻米的实际产量具有十分重要的经济价值。Brown rice rate is one of the indexes for rice milling quality measurement .In this study ,the inherit-ance of brown rice rate was characterized using 119 chromosome single segment substitution lines ( CSSSLs ) derived from Nipponbare and Guangluai 4 .One-way ANOVA and Dunnett′s test were employed to compare the difference between CSSSLs and the recurrent parent Guangluai 4.We detected 2 QTL(P≤0.001) for brown rice rate which were primarily mapped on chromosome 6 and 9 by substitution mapping .Both of the allele from donor parent Nip-ponbare had positive effects .The additive effects of 2 QTL were 1.49% and 1.32% respectively, with additive effect percentages 1.89%and 1.68%.These results provide useful information for exploring and using new brown rice rate related QTL,and also have important economic value for increasing brown rice rate and actual production of rice.

关 键 词：水稻 糙米率 数量性状位点 染色体单片段代换系 代换作图 

分 类 号：Q511.03[生物学—生物化学]