利胆止痛片对α-萘异硫氰酸致急性肝损伤的保护作用  

Protective Effect of Li dan zhi tong Tablets on Acute Hepatic Iniury Induced by α-Naphthyl Isothiocyanate

作　　者：闫俊岭 郭丽蓉 徐松 张信岳[2] 张立群 陈爱君[2] 

机构地区：[1]云南白药集团股份有限公司云南白药研究院,云南昆明650500 [2]浙江省医学科学院药物研究所,浙江杭州310013

出　　处：《昆明医科大学学报》2013年第7期30-33,共4页Journal of Kunming Medical University

摘　　要：目的研究利胆止痛片对小鼠急性肝损伤的保护作用.方法采用α-萘异硫氰酸(ANIT)造成小鼠急性肝损伤模型,观察利胆止痛片[1.5、0.75、0.375 g(浸膏)/kg体重]对肝损伤小鼠的体重、肝脏系数、肝功能指标、肝组织中丙二醛(MDA)与超氧化物歧化酶(SOD)的影响,并对肝脏组织进行病理学观察.结果利胆止痛片(1.5、0.75、0.375 g/kg)能明显改善ANIT所致急性肝损伤小鼠体重的减轻,改善肝功能,减低肝组织中MDA含量、提高SOD活性,改善肝组织病理损伤,提示利胆止痛片对急性肝损伤具有良好的保护作用.结论利胆止痛片对ANIT所致小鼠急性肝损伤具有明显的保护作用.Objective To study the protective effect of Li dan zhi tong tablets on mice model with the acute hepatic lesion induced by α-Naphthyl isothiocyanate(ANIT).Methods The rats were randomly divided into six groups,namely,the normal group,the ANIT group,the Xiao yan li dan tablets group and the Li dan zhi tong tablets group(1.5,0.75,0.375 g extractum/kg body weigh)t.The mice model with the acute hepatic lesion was established by ANIT after 8 days by giving corresponding drugs to each group.Body weights and liver coefficients of the mice were determined 48 hours later,and such hepatic functions as glutamic-pyruvic transaminase(ALT),glutamic-oxaloacetic transaminase(AST),alkaline phosphatase(ALP),total bilirubin(TBIL),direct bilirubin(DBIL) and total bile acid(TBA) were examined.Also the levels of malondialdehyde(MDA) and superoxide disnutase(SOD) in liver tissue homogenates were determined by observing the liver tissues pathologically under the microscope.Results Compared with ANIT group,body weights of the mice in Li dan zhi tong tablets group(1.5,0.75,0.375 g extractum/kg body weigh)t were significantly restored,the hepatic functions were improved and the tissue pathological damages were alleviated,which indicated that the levels of MDA were markedly lowered in Li dan zhi tong tablets treatment group with the activities of SOD being markedly increased.Conclusion Li dan zhi tong tablet has preferable preventive and therapeutic effects on the mice with acute liver injure induced by ANIT.

关 键 词：利胆止痛片 急性肝损伤 肝功能 丙二醛 超氧化物歧化酶 

分 类 号：R965[医药卫生—药理学]