通用型辅助性T细胞表位增强了猪瘟病毒合成肽抗原的免疫原性  

PROMISCUOUS HELPER T-CELL EPITOPE ENHANCES THE IMMUNOGENICITY OF SYNTHETIC PEPTIDE ANTIGEN OF CLASSICAL SWINE FEVER VIRUS

作　　者：李国新[1] 郑宝亮[2] 周艳君[1] 于海[1] 童武[1] 童光志[1] 

机构地区：[1]中国农业科学院上海兽医研究所,上海200241 [2]郑州牧业工程高等专科学校生物工程系,郑州450011

出　　处：《中国动物传染病学报》2013年第2期1-6,共6页Chinese Journal of Animal Infectious Diseases

基　　金：上海市科技兴农重点攻关项目[沪农科攻字(2009)第5-4号];公益性行业(农业)科研专项(201203039)

摘　　要：为了探讨通用型辅助性T细胞表位对猪瘟病毒人工合成短肽抗原免疫原性的影响,本研究合成了含有猪瘟病毒E2蛋白线性中和表位的短肽B,合成了含有通用型辅助性T细胞表位的短肽Th-B。同时,为了探讨免疫刺激基序对人工合成短肽抗原免疫原性的影响,合成了含有免疫刺激序列的IS-Th-B。将上述3种合成肽与弗氏佐剂混合乳化后,免疫兔子,比较3种不同的抗原肽诱导的抗体水平,并用猪瘟弱毒对免疫兔子进行攻击,比较抗原肽的免疫保护效果。结果显示,Th-B和IS-Th-B诱导了相当的抗体水平,但显著高于抗原肽B诱导的抗体水平。Th-B和IS-Th-B免疫延迟但没有阻止兔定型热的产生,而抗原肽B免疫兔和未免疫兔一样发生了明显的定型热反应。本研究表明,免疫刺激基序对本研究中的短肽诱导的抗体应答没有产生明显影响,但通用型辅助性T细胞表位增强了短肽抗原的免疫原性。The present study was designed to illustrate the effect of promiscuous helper T-cell epitope on the immunogenicity of three synthetic peptide antigens of Classical swine fever virus(CSFV) E2 protein.Peptide B contained a linear neutralizing epitope and peptide Th-B linked a promiscuous helper T-cell epitope to peptide B.Similarly,peptide IS-Th-B linked an immunostimulatory peptide to peptide Th-B.These peptides were emulsified in complete Freund’s adjuvant for the first immunization and in incomplete Freund’s adjuvant for a booster immunization in rabbits.Peptides IS-Th-B and Th-B induced almost the same antibody levels but significantly higher than peptide B did.Immunization with peptides IS-Th-B and Th-B delayed typical rabbit fever that showed up later.However,rabbits immunized with peptide B developed typical fever as seen in control rabbits.Results indicated that the promiscuous helper T-cell epitope enhanced the immunogenicity of synthetic peptide antigens while immunostimulatory peptide did not.

关 键 词：猪瘟病毒 通用型辅助性T细胞表位 线性中和表位 合成肽 

分 类 号：S852.651[农业科学—基础兽医学]