姜黄素对缺血/再灌注大鼠APE/Ref-1表达及细胞凋亡的影响  

Effect of curcumin on APE / Ref-1 expression and apoptosis in rats with cerebral ischemia-reperfusion injury

作　　者：王智[1] 薛荣亮[2] 赵红霞[2] 高慧[2] 魏欣[2] 

机构地区：[1]西安交通大学口腔医院,西安710004 [2]西安交通大学医学院第二附属医院

出　　处：《山东医药》2013年第37期1-3,11,共4页Shandong Medical Journal

基　　金：国家自然科学基金资助项目(30571790;81071070)

摘　　要：目的观察姜黄素对大鼠全脑缺血/再灌注损伤后海马神经元凋亡与DNA修复蛋白APE/Ref-1表达的影响,探讨其对脑缺血损伤后的保护作用。方法将144只雄性SD大鼠随机平均分为假手术组(SO组)、缺血/再灌注组(IR组)和姜黄素干预组(CU组)各48只。建立大鼠全脑缺血/再灌注模型,CU组于再灌注后即刻经腹腔注射姜黄素。分别于再灌注后2、6、12、24、48、72 h处死大鼠,提取大鼠脑组织。原位末端标记法检测海马CA1区的细胞凋亡情况;免疫组化SABC法检测APE/Ref-1的表达。结果 SO组神经元细胞凋亡较少,IR组凋亡的神经元细胞于再灌注6 h开始增多,48 h细胞凋亡率达到最高。与SO组比较,IR组细胞凋亡率增高(P<0.01)。CU组在再灌注6 h后的各时点神经元细胞凋亡较IR组少(P均<0.01)。SO组APE/Ref-1阳性细胞在各时点较多;IR组于再灌注后2 h APE/Ref-1阳性细胞开始出现明显减少,一直持续至72 h,与SO组比较有统计学差异(P均<0.01);CU组APE/Ref-1阳性细胞率各时点与IR组比较均有统计学差异(P均<0.01)。结论姜黄素发挥脑保护作用的机制可能与减缓DNA修复蛋白APE/Ref-1表达的下降和阻止脑缺血/再灌注损伤区神经元细胞的凋亡有关。Objective To observe the effect of curcumin on the apoptosis of neuron in hippocampus and the changes of DNA repair proteins APE / Ref-1 after global cerebral ischemia-reperfusion injury in rats and to explore the protective effect of curcumin on brain. Methods A total of 144 male SD rats were randomly divided into 3 groups,48 in each group: the sham operation group( SO group),ischemia-reperfusion group( IR group) and curcumin intervention group( CU group). The global cerebral ischemia-reperfusion rat models were established,and then to inject curcumin into the CU group immediately. Rats were executed to obtain the hippocampus tissues at 2 h,6 h,12 h,24 h,48 h and 72 h after cerebral ischemia-reperfusion( CIR) respectively. The apoptosis was detected by TUNEL and the expression of APE / Ref-1 proteins by immunohistochemical SABC method. Results Apoptotic neurons in the SO group were few,but in the IR group,the rate of apoptosis increased obviously at 6 h after CIR,reached the peak at 48 h. Compared with the SO group,the rate of apoptosis increased in the IR group and with a significant difference( P < 0. 01). The rate of apoptosis in the CU group was lower than that in the IR group at different time points except at 2 h,which was meaningful in statistics( all P < 0. 01). The positive expression of APE / Ref-1 in the SO group was high at different time points,however,the positive expression of the APE / Ref-1 in the IR group started to decrease at 2 h after CIR,and the decrease lasted until 72 h. Compared with the SO group,a significant difference was found( all P < 0. 01). The positive expression rate of APE / Ref-1 in the CU group at different time points was significantly different as compared with that of the IR group( all P < 0. 01). Conclusion The curcumin has the protective effect on the cerebrum,whose mechanism may be associated with slowing down the decrease of APE / Ref-1 expression,and preventing the apoptosis of neurons in cerebral ischemia-reperfusion injury region.

关 键 词：再灌注损伤 APE REF-1 姜黄素 大鼠 脑 

分 类 号：R651.1+5[医药卫生—外科学]