转铁蛋白受体介导载紫杉醇纳米粒对结肠癌细胞的靶向性研究  

Research of transferrin receptor mediated nanoparticles loaded paclitaxel targeting to colon cancer cell

作　　者：钟玲[1,2] 李思齐[3] 杨蔚[2] 曾蓉[2] 毛琴[2] 陈嘉勇[3] 

机构地区：[1]昆明医科大学,云南昆明650500 [2]云南省第一人民医院急诊科,云南昆明650032 [3]昆明医科大学第二附属医院急诊科,云南昆明650101

出　　处：《中国生化药物杂志》2014年第5期23-25,29,共3页Chinese Journal of Biochemical Pharmaceutics

基　　金：云南省应用基础研究重点项目(2011FA029);云南省科技厅﹣昆明医科大学联合专项基金项目(2010CD165)

摘　　要：目的制备转铁蛋白受体特异结合肽T7修饰载紫杉醇纳米粒(T7-NPs-PTX),研究其对结肠癌RKO细胞的靶向抑制作用。方法采用薄层法利备纳米粒共聚焦显微镜观察结肠癌RKO细胞和血管内皮HUVEC细胞对T7-NPs-PTX的摄取情况。MTT实验研究T7-NPs-PTX对结肠癌RKO细胞的毒性;流式细胞仪检测T7-NPs-PTX对结肠癌RKO细胞的凋亡诱导作用。构建RKO细胞肿瘤球模型,研究T7-NPs-PTX对肿瘤球的生长抑制能力。构建结肠癌裸鼠异位模型,研究T7-NPs-PTX对裸鼠肿瘤生长抑制作用。结果 RKO细胞对经过T7修饰后的T7-NPs-PTX摄取显著增加,但HUVEC细胞对T7-NPs-PTX的摄取无显著变化。T7-NPs-PTX对结肠癌RKO细胞的增殖抑制作用和细胞凋亡诱导作用显著强于NPs-PTX和PTX溶液。结论经过T7修饰后能够增强纳米粒与结肠癌RKO细胞的亲和力,T7-NPs-PTX是一种潜在、高效的结肠癌靶向给药系统。Objective To prepare T7 peptide conjugated nanoparticles loaded aclitaxel(T7-NPs-PTX),evaluate its properties and study its effect on colon cancer. Methods Nanoparticles were prepared by nanopreparation method. The cellular uptake of RKO cell and HUVEC cell in vitro were observed by confocal laser. The anti-proliferation efficiency of T7-NPs-PTX was evaluated by MTT assay. Tumor spheroids test was used to evaluate antitumor ability of T7-NPs-PTX. RKO cell were xenografted into athymic mice to establish the animal model,which were used to evaluate the effect of anticancer. Results The result demonstrated that T7-NPs uptaken by RKO cell was higher than that of LP. The MTT assay and the inhibition of tumor spheroids test in vitro confirmed strong inhibitory effect of T7-NPs-PTX. Conclusion T7-NPs-PTX is easy to prepare and it is a potential delivery system for the treatment of colon cancer.

关 键 词：转铁蛋白受体 紫杉醇 药物靶向 纳米粒 

分 类 号：R735.3[医药卫生—肿瘤]