2-DG联合5-Fu对胃癌细胞增殖和凋亡影响的实验研究  

Effects of 2-deoxy-D-glucose and 5-Fu on proliferation and apoptosis of human gastric cancer MGC-803 cells

作　　者：章斐然[1] 李威[1] 郑仰[1] 陈君填[1] 

机构地区：[1]汕头大学医学院第一附属医院胃肠外科,汕头515040

出　　处：《消化肿瘤杂志（电子版）》2013年第1期48-51,共4页Journal of Digestive Oncology(Electronic Version)

基　　金：广东省科技计划项目(2011B031800189)

摘　　要：目的探讨糖酵解抑制剂2-脱氧-D-葡萄糖(2-deoxy-D-glucose,2-DG)与5-氟尿嘧啶(5-Fu)单独及联合应用对人胃癌细胞MGC-803增殖及凋亡的影响。方法将2-DG(10 mmol/L)、及5-Fu(5.0μg/L)单药及联合用药作用于人胃癌细胞MGC-803,MTT法检测各组细胞的生长抑制率,流式细胞仪检测各组细胞凋亡情况。结果联合用药组(2-DG+5-Fu)对MGC-803增殖抑制率为(70.93±3.84)%,显著高于2-DG组(30.61±1.72)%和5-Fu组(47.08±4.34)%,差异有统计学意义(P<0.05)。联合用药组肿瘤细胞凋亡率为(38.96±2.62)%,2-DG、5-Fu单药组肿瘤细胞凋亡率分别为(15.70±1.42)%、(25.56±2.64)%,联合用药组分别与单药组比较,差异有统计学意义(P<0.05)。结论 2-DG能有效抑制人胃癌细胞MGC-803细胞生长增殖,并诱导其凋亡;2-DG与5-Fu联合应用抗肿瘤作用明显增强。Objective To investigate the effects of 2-deoxy-D-glucose( 2-DG) or 5-Fu alone and the combination of both on the proliferation and apoptosis of human gastric cancer MGC-803 cells.Methods Human gastric cancer MGC-803 cells were treated with 2-DG(10 mmol / L) or 5-Fu(5.0 μg / L)alone,or combination of both. The inhibitory effect on the proliferation of MGC-803 cells was estimated by MTT method.Apoptotic rate was assayed by flow cytometry. Results The proliferation inhibitory rate of tumor cells was(70.93±3.84)% in the combination group, significantly higher than(30.61±1.72)%and(47.08±4.34)% respectively in single 2-DG and 5-Fu groups(P<0.05). The apoptotic rate of tumor cells was(38.96±2.62)% in the combination group, significantly higher than(15.70±1.42)%,(25.56±2.64)% respectively in single 2-DG、5-Fu groups(P<0.05). Conclusions 2-DG can inhibit proliferation and induce apoptosis of human gastric cancer MGC-803 cells.The combination of 2-DG and 5-Fu can increase such anti-tumor effect.

关 键 词：2-脱氧-D-葡萄糖 胃癌 增殖 细胞凋亡 

分 类 号：R735.2[医药卫生—肿瘤]