兔多杀性巴氏杆菌16S rRNA PCR检测方法的建立和应用  

Establishment and Application of the PCR Detection Method on Rabbit Pasteurella Multocida 16S rRNA

作　　者：庞安娜[1] 刘燕[1] 韦强[1] 肖琛闻[1] 鲍国连[1] 季权安[1] 

机构地区：[1]浙江省农科院畜牧兽医研究所,杭州310021

出　　处：《中国养兔》2011年第8期4-7,共4页Chinese Journal of Rabbit Farming

基　　金：公益性行业科研专项"肉兔高效饲养研究与示范"(nyhyzx07-040);国家现代农业兔产业体系建设疾病预防与控制岗位(nycytx-44-3-2)

摘　　要：以兔多杀性巴氏杆菌的高度保守的16S rRNA为靶基因,参考已公布的多杀性巴氏杆菌的16SrRNA基因设计1对特异性引物,优化PCR反应条件,建立了兔多杀性巴氏杆菌PCR快速检测方法。该PCR方法的敏感性达到60cfu/mL,使用建立的PCR方法扩增兔多杀性巴氏杆菌标准株和分离株均能扩增出643bp的目的片段,扩增兔大肠杆菌、支气管败血波氏杆菌结果为阴性,证明本试验所建立的兔多杀性巴氏杆菌病原PCR检测方法敏感、特异、可靠。Highly conserved 16S rRNA of rabbit Pasteurella multocida was as target gene,and according to Pasteurella multocida 16S rRNA sequence in GeneBank,a pair of specific primers was designed.The conditions of PCR reaction were optimized,and the PCR rapid detection method on rabbit Pasteurella Multocida was established.The minimum detection amount was 60 cfu/mL.Using the new established method,a specific fragment of 643 bp could be amplified from standard and isolated strains of Pasteurella multocida,but not Escherichia coli.and Bordetella bronchiseptica.The results showed that the PCR method established in this experiment is sensitive,specific and reliable on the detection of rabbit pasteurellosis.

关 键 词：兔 多杀性巴氏杆菌 PCR 16S RRNA 

分 类 号：S858.291[农业科学—临床兽医学]