Spring constant regulate selectin-ligand bond dissociation  

作　　者：Yan Zhang Ganyun Sun Shouqin L Mian Long 

机构地区：[1]Center of Biomechanics and Bioengineering and Key Laboratory of Microgravity,Institute of Mechanics,Chinese Academy of Sciences

出　　处：《医用生物力学》2013年第S1期95-96,共2页Journal of Medical Biomechanics

基　　金：supported by National Natural Science Foundation of China grants 10902117, 31230027,30730032,and 10332060

摘　　要：Forced dissociation of selectin-ligand complex is crucial to such biological processes as leukocyte recruitment,thrombosis formation,as well as tumor metastasis^[1].Although several assays and techniques,e.g.,dynamic force spectroscopy（DFS）,have been applied to probe the complex at single-bond level,the discrepancies in the loading rate dependence of bond rupture force were found in the assays,presumably due to the different pathways in energy landscape and binding kinetics of molecular complexes^[2].However,the underlying mechanisms remain unclear.Here an optical trap（OT）assay was used to quantify the bond rupture at r_f≤20 pN/sForced dissociation of selectin-ligand complex is crucial to such biological processes as leukocyte recruitment,thrombosis formation,as well as tumor metastasis[1].Although several assays and techniques,e.g.,dynamic force spectroscopy（DFS）,have been applied to probe the complex at single-bond level,the discrepancies in the loading rate dependence of bond rupture force were found in the assays,presumably due to the different pathways in energy landscape and binding kinetics of molecular complexes[2].However,the underlying mechanisms remain unclear.Here an optical trap（OT）assay was used to quantify the bond rupture at rf≤20 pN/s with

关 键 词：SELECTIN DISSOCIATION RUPTURE kinetics landscape metastasis REGULATE quantify LEUKOCYTE unclear 

分 类 号：R318.0[医药卫生—生物医学工程]