固定化酵母高浓度乙醇多级连续发酵控制策略研究  

Novel strategies for multistage continuous very high gravity ethanol fermentation using immobilized yeast cells

作　　者：俞建良[1,2] 吉海瑞[1] 张栩[1] 谭天伟[1] 

机构地区：[1]国家能源生物炼制研发中心,北京化工大学,北京100029 [2]中粮集团生化能源事业部,北京100020

出　　处：《中国酿造》2013年第S1期25-28,共4页China Brewing

基　　金：国家"863"计划项目(2013AA050702;2012AA022304)

摘　　要：为了解决高浓度乙醇连续发酵过程中酵母受抑制严重,系统稳定运行周期短的难题,研究了罐体轮换和二氧化碳气提两种策略在固定化细胞高浓度乙醇多级连续发酵中的应用。实验结果表明,气提可以带出第4级和第5级罐体中5.5%~10%的乙醇,降低了乙醇对酵母细胞的毒害作用,提高了固定化细胞的存活率,体系的稳定运行周期从21d延长至35d。同时,乙醇生成速率从单一罐体轮换控制时的1.98g/(L·h)提高至罐体轮换结合气提控制时的2.46g/(L·h)~2.62g/(L·h)。In this study,an innovative approach called reactor rotation and self-gas stripping was applied to reduce the toxic effect of ethanol and residual sugars on yeast cell,so that to prolong the steady running time of a five-stage continuous ethanol fermentation system.Due to the disturbance caused by gas stripping,reactor rotation coupled with self-gas stripping carried out 5.5%~10% of the total ethanol produced in stage 4(S4) and stage 5(S5) and increased the immobilized cell viabilities,which prolonged the steady state period from 21d to 35d.Meanwhile,ethanol productivities increased from 1.98g/(L·h)(reactor rotation alone) to 2.46g/(L·h)~2.62g/(L·h)(reactor rotation coupled with self-gas stripping).

关 键 词：乙醇 连续发酵 固定化 罐体轮换 气提 

分 类 号：TQ223.122[化学工程—有机化工]