基因芯片技术研究虚热证大鼠阴虚内热的分子机制  

Research on Molecular Mechanism of"Yin Asthenia with Internal Heat"in Asthenia Hot Syndrome Rats by Gene Chip Technology

作　　者：韩冰冰[1] 王世军[2] 

机构地区：[1]山东中医药大学基础医学院,山东济南250355 [2]山东中医药大学国际教育学院,山东济南250355

出　　处：《四川中医》2013年第8期37-40,共4页Journal of Sichuan of Traditional Chinese Medicine

基　　金：国家重点基础研究发展计划(973计划)(编号:2007CB512601;2013CB531803)

摘　　要：目的:通过基因芯片技术研究虚热证大鼠肝全基因表达谱的改变,阐释虚热证大鼠"阴虚内热"的分子机制。方法:使用中药复方附子、肉桂、干姜建立虚热证大鼠模型,应用基因芯片检测各组大鼠肝脏基因表达,筛选差异表达基因,进行基因功能分类注释,荧光定量PCR验证芯片结果。结果:虚热模型组与空白对照组比较有99条基因差异表达,主要涉及防御应答,固醇代谢等功能。结论:虚热证阴虚内热的分子机制可能与刺激应答相关基因及固醇代谢相关基因两类基因的异常表达相关。To explain molecular mechanism of'Yin Asthenia with Internal Heat'in asthenia hot syndrome rats by analysis the whole gene expression profile of the liver in rats with asthenia hot syndrome.Methods: The Asthenia hot syndrome rats models were induced by compound preparation of Traditional Chinese Medicine of Aconitum carmichaeli,Cinnamomi Cortex,Rhizoma Zingiberis.The liver gene expression in each group was detected by gene chip.We selected the differential expression genes and conducted the significant analysis on the genetic function of differential genes.A part of genes were selected to test the accuracy of results by RT-PCR.Results: As compared to the control group,in asthenia hot model group there were 99 strips of differential expression gene,mainly about function of defense response and sterol metabolic process.Conclusion: The abnormal expression of genes about defense response and sterol metabolic process in asthenia hot syndrome rats is possibly related to the substance foundation of asthenia hot syndrome.

关 键 词：基因芯片 虚热证 阴虚内热 

分 类 号：R228[医药卫生—中医基础理论]