人参皂甙Rg3对小鼠肝癌H22腹水瘤抗血管生成作用的研究  

Antlangingenesis of panaxoside-Rg3 on ascitic-fluid type helmtocarcinoma H22 in mice

作　　者：井小会[1] 吴会战[1] 张灿珍[2] 

机构地区：[1]河南省平顶山市第一人民医院肿瘤科,河南,平顶山,467000 [2]云南省肿瘤医院肿瘤内科

出　　处：《中国医师杂志》2008年第8期-,共3页Journal of Chinese Physician

摘　　要：目的 研究人参皂甙Rg3治疗小鼠恶性腹腔积液的抗血管生成作用.方法 50只雌、雄各半昆明种小鼠随机分为5组:Ⅰ组生理盐水组(0.9%NS);Ⅱ组顺铂组(DDP 0.5 mg/kg);Ⅲ组低剂量人参皂甙Rg3组(LPD 0.3 mg/kg);Ⅳ组中剂量人参皂甙RS3组(MPD 1.0 mg/ks);Ⅴ组高剂量人参皂甙Rg3组(HPD 3.0ms/ks).所有小鼠肝癌H22腹水瘤模型建立后24 h开始分别腹腔注射0.2 ml/只治疗,1次/d,共14 d.治疗结束后24 h,处死各组小鼠,应用酶联免疫吸附法检测腹水及血清中血管内皮生长因子(VEGF),免疫组织化学法计数腹膜瘤结节微血管密度(MVD),电镜观察人参皂甙Rg3中刺量组与生理盐水组腹腔内肿瘤细胞及腹膜瘤结节新生血管的形态学变化.结果 人参皂甙Rg3各剂量组随着用药剂量的加大,小鼠腹水及血清中VEGF值、腹膜瘤结节MVD下降(P＜0.05),且均较生理盐水组、DDP组下降(P＜0.05).电镜观察人参皂甙Rg3中剂量组较生理盐水组腹水中凋亡和坏死瘤细胞居多;腹膜瘤结节微血管基底膜平滑完整.结论 人参皂甙Rg3通过下调荷瘤小鼠体内VEGF,降低微血管的通透性和抑制腹膜微血管形成,从而抑制恶性腹腔积液的形成.这为临床应用提供了实验依据.Objective To observe the antiangiogenesis of panaxoside-Rg3 on mice intraperitoneally implanted with ascites tlnnor cells.Methods 25 female and 25 male Kunming mice were random divided into five groups:Group I injected with normal saline (0.9%NS),Group Ⅱ with cisplatin(DDP 0.5mg/kg),Group Ⅲ,with low-dose panaxoside-Rg3(LPD 0.3mg/kg),Group Ⅳ with middle-dose panaxoside-Rg3(MPD 1.0mg/kg),Group Ⅴ with high-dose panaxoside-Rg3(HPD 3.0mg/kg).Experimental ascitic hepatocarcinoma of H22 lines model were successfully established among all groups,and 24 hours later intraperitoneal infusion of 0.2ml medicines was given to each mouse once every day for 14 days.24 hours after the over of the treatment,all mice were executed.Enzyme linked immunosorbent assay(ELISA)method was used to detectt he different VEGF level in the ascites and serum of all groups and expressions of micmvessel density (MVD)of peritoneum tumor node was calculated by immunohistochemical staining with CD31 antibody.Morphological of tumor cell in abdominal cavity and new vascular in peritoneum tumor node were observed by transmission electron microscope.Results With the increase of concentration of panaxoside-Rg3,expressions of the VEGF level of the ascites and the serum and MVD in peritoneum dropped(P＜0.05)and decreased more than that in the NS group and the DDP group(P＜0.05).Morphological changes of tumor ceHs in panaxoside-Rg3 group were observed with electronic scope,more apoptosis and necrosis cells were found.Capillary vessel basal lamina was smoothing.Condusion Panaxoside-Rg3 decreases the permeability of mierangium and inhibit the neovascula-rization of peritoneum by decreasing the VEGF level,accordingly,panaxoside-Rg3 inhibit the formation of malignant ascites.This would offer foundation of theory for clinical application.

关 键 词：人参皂甙/药理学 肝肿瘤 腹水 新生血管化 病理性 血管内皮生长因子类 

分 类 号：R735.7[医药卫生—肿瘤]