C-myc反义寡核苷酸对人胆管癌细胞增殖和侵袭力的影响  

The effect of c-myc antisense oligodeoxynucleotide on the proliferation and invasion of QBC939 cells

作　　者：吴一飞[1] 李灼日[2] 毛先海[2] 吴金术[2] 

机构地区：[1]南华大学,湖南,衡阳,421500 [2]湖南省人民医院肝胆外科

出　　处：《中国医师杂志》2008年第12期-,共3页Journal of Chinese Physician

摘　　要：目的 探讨C-myc反义寡核苷酸(ASODN)对人胆管癌QBC939细胞增殖和侵袭力的影响.方法 合成C-mycASODN,并将其转染QBC939细胞;四甲基噻唑蓝(MTT)实验检测C-myc ASODN抑制QBC939细胞增殖的影响;Transwell法检测C-myc ASODN抑制QBC939细胞侵袭力的影响.结果 ASODN组细胞的存活率低于空白对照组(P<0.05);ASODN组较空白对照组的细胞穿透数目下降(P<0.01);空载体组细胞的存活率及细胞穿透数目较空白对照组差异无统计学意义(P>0.05).结论 C-myc ASODN能抑制QBC939细胞的增殖和侵袭力.Objective To investigate the effect of c-myc ASODN on the proliferation and invasion of human bile duct carcinoma cell line QBC939. Methods QBC939 cells was conventionally cultured. C-myc ASODN was designed and transfected into QBC939 cell line. MTT assay and transwell experiment were used to study cell proliferation and invasion of QBC939 cells. Results MTT assay showed that cell survival rate in ASODN group was significantly lower than that in blank group(P < 0.05). Transwell experiment showed that the num-ber of cells penetrated in ASODN group was significantly lower than that in blank group(P<0.01). The cell survival rate and the number of cells penetrated in vechicle group had no difference with blank comparison group(P>0.05). Conclusions C- myc ASODN can inhibit the proliferation and invasion of QBC939 cells.

关 键 词：基因 myc 寡核苷酸类 反义 胆管肿瘤 细胞增殖 肿瘤侵润 

分 类 号：R735.8[医药卫生—肿瘤]