一组与焦虑症相关的蛋白质组指纹的研究报告  

Preliminary study on the possible correlation between anxiety and proteomic fingerprint

作　　者：王清馨[1] 姚璧[1] 郑丽君[1] 赵丽华[1] 王鑫[1] 裴毅[1] 

机构地区：[1]山西省肿瘤医院老年病科,030013

出　　处：《肿瘤研究与临床》2008年第7期442-444,共3页Cancer Research and Clinic

基　　金：山西省留学生基金(0571)

摘　　要：目的 初步分析新发现的质荷比(M/Z)15 000+H～16 800+H范围内蛋白质组指纹表达与焦虑症的相关性.方法 2004年4月至2006年12月,对221例肿瘤患者进行了观察,全部采用焦虑自评量表(SAS)进行评估,根据评分标准分为焦虑症组和无焦虑组,应用蛋白质飞行时间质谱技术(SELDI-TOF-MS)检测患者血清,获得指纹图进行分析.结果 采用SAS评估221例患者,其中焦虑症患者49例,无焦虑患者172例,在焦虑症组患者的血清中捕获到M/Z为15 000+H～15 380+H和15 700+H～16 800+H的质谱峰簇,与SAS评估的符合率为75.51%,其中,M/Z为15 700+H～16 800+H出现频度最高,其次是M/Z为15 000+H～15 380+H和15 700+H～16 800+H的双峰簇指纹,M/Z为15 000+H～15 380+H峰簇指纹出现频度最少.在SAS评估为无焦虑的患者血清中也捕获到上述指纹图谱,二者比较除丰度有差异外,其质荷比是一致的.结论 M/Z为15 000+H～16 800+H处的峰簇为焦虑症相关蛋白质指纹.Objective A preliminary study on a new discovered proteomic fingerprint correlated with anxiety disorders, which M/Z range from 15000 to 16800. Methods 221 cases of neoplasm patients from April of 2004 to now were divided into anxious group and non-anxious group according to their scores using self-rating anxiety scale (SAS), and all the patients' sera were detected utilizing SELDI-TOF-MS. The data were analyzed with supported softwares. Results Compared the mass spectra of anxious group(n=49) and non-anxious group (n=172), 2 clusters were captured which M/Z range from 15 000 to 15 380 and 15 700 to 16 800 respectively. The coincidence with SAS was 75.51%. The M/Z range from 15 700 to 16 800 was the most frequent, then the second range from 15 000 to 15 380 and 15 700 to 16 800 which was doublet cluster, the least range from 15 000 to 15 380. The same fingerprints were captured in non-anxious group and their intensity was different. Conclusion The M/Z range from 15 000 to 16 800 was protein fingerprint correlated with anxiety disorders.

关 键 词：肿瘤 焦虑症 蛋白质组 表现焦虑量表 SELDI技术 

分 类 号：R74[医药卫生—神经病学与精神病学] R73[医药卫生—临床医学]