大肠癌hTERT、SYK分子边界的临床研究  

The clinic study on molecular margin in colorectal cancer

作　　者：朱挺[1] 张乐鸣[1] 周建良[1] 柯孔亮[1] 

机构地区：[1]浙江省宁波市第一医院肿瘤科,315010

出　　处：《肿瘤研究与临床》2008年第10期682-684,共3页Cancer Research and Clinic

摘　　要：目的 探讨大肠癌及其切缘组织中hTERT、SYK活性表达、存在的分子边界及其与手术切缘的安全性关系.方法 采用SABC、Supervion二步免疫组织化学法分别检测20例大肠癌标本及其远近端癌旁各1、2、3、4、5 cm组织中hTERT、SYK活性的表达.结果 hTERT在大肠癌的阳性表达率为80.00%,癌旁3、4、5 cm组织中其阳性表达率为0;hTERT的阳性表达从癌中心向癌旁1、2、3 cm组织逐渐递减(P<0.01).SYK在大肠癌的阳性表达率为10.00%,SYK的阳性表达从癌中心向癌旁1、2、3 cm组织逐渐递增(P<0.05).结论 在大肠癌周边组织中存在着分子边界,根据hTERT和SYK的表达情况,3 cm可以定义为大肠癌根治手术安全切除的分子边界.Objective To study the human telomerase reverse transcriptase (hTERT) and spleen tyrosine kinase (SYK) activity expression and molecular margin in the tumor center and peripheral tissues of patients with colorectal cancer, and to explore the security of tumor surgical margins. Methods The expressions of hTERT and SYK were examined by SABC and Supervisan immunohistochemistry in 20 specimens of colorectal cancer and tissues 1 cm, 2 cm, 3 cm, 4 cm and 5 cm, away from proximal and distal margin of the tumor respectively. Results The positive expression rate of hTERT in colorectal cancer was 80.00 %, the rate in tissues 3 cm, 4 cm and 5 cm away from the tumor margin was 0. The positive expression rate of hTERT in tissues from the cancer center to 3 cm away from the tumor margin was gradually diminishing (P<0.01). The positive expression rate of SYK in colorectal cancer was 10.00 %, the positive expression rate of SYK in tissues from the cancer center to 3 cm away from the tumor margin was gradually increased (P<0.05). Conclusion There is molecular margin around colorectal cancer tissues. Considering hTERT and SYK expressions, 3 cm can be defined as the safe molecular margin for the radical operation of colorectal cancer.

关 键 词：肠肿瘤 端粒 末端转移酶 蛋白酪氨酸激酶类 分子边界 

分 类 号：R735[医药卫生—肿瘤]