戊型肝炎病毒树鼩感染模型的初步研究  

Primary Study on Experimental Infection of Tree Shrew(Tupaia belangeri) by Swine Hepatitis E virus

作　　者：蒋雷[1] 尹革芬[1] 舒相华[1] 李进涛[2] 胡映开[1] 杨贵树[1] 李文贵[1] 

机构地区：[1]云南农业大学动物科学技术学院,云南昆明650201 [2]昆明医科大学实验动物学部,云南昆明650550

出　　处：《云南农业大学学报》2013年第2期186-189,共4页Journal of Yunnan Agricultural University

基　　金：国家自然科学基金项目(31160495)

摘　　要：戊型肝炎病毒(HEV)研究多以灵长类动物为感染模型。由于灵长类动物价格昂贵,实验条件的控制和饲养管理较困难等原因,严重阻碍了HEV研究进展。本研究对应用树鼩(Tupaia belangeri)建立HEV感染模型的可行性进行了探索。从猪群中分离、鉴定HEV,经静脉注射途径感染树鼩,按计划采集粪便、血液以及器官组织,应用巢式RT-PCR检测树鼩感染情况,观察感染后树鼩各器官组织病理学病化。结果发现,感染7 d后血液、粪便、肝脏、小肠等样品可检测出HEV RNA,病毒血症持续约2周。组织病理学观察结果,人工感染HEV的树鼩肝脏可见静脉窦淤血、多发性淋巴细胞浸润、库普弗细胞增多等病变。研究结果表明树鼩对HEV具有易感性,感染后肝脏出现病毒性肝炎的病理学变化,具有作为戊型肝炎的感染模型的潜在价值。At present,nonhuman primates are mostly frequently used in Hepatitis E virus(HEV)research.However,high cost,difficulties in handling,manipulating and housing severely restrict their use in large numbers in research.To analyze the possibility of using tree shrews(Tupaia belangeri) to establish infection model of HEV,tree shrews were experimentally inoculated with swine HEV via intravenous.Sera and feces samples were collected,two tree shrews from test group were necropsied weekly,pathological changes were recorded and tissue samples collected for further investigation.Study results found that HEV RNA could be detected in feces,blood,liver and intestine 7 days post inoculation.Histopathological changed as kupffer cells increased,multiple lymphocyte infiltration and congestion in hepatic sinusoid were observed in hepatic tissues.These results indicate that tree shrews could potentially use as an ideal animal model for the study of HEV.

关 键 词：戊型肝炎病毒 感染 树鼩 

分 类 号：R-332[医药卫生]