Distribution of Salmonella paratyphi A outer membrane protein X gene and immune-protective effect related to its recombinant expressed products  

作　　者：李明雷 

机构地区：[1]Yiwu Centr Hosp

出　　处：《China Medical Abstracts(Internal Medicine)》2014年第1期13-13,共1页中国医学文摘（内科学分册（英文版）

摘　　要：Objective To determine the distribution and sequence conservation of outer membrane protein X(ompX)gene in Salmonella paratyphi A isolates as well as the immunogenicity and immono-protection of ompX gene products.Methods OmpX gene in Salmonella paratyphi A isolates was detected by PCR and the amplification products were sequenced after the T-A cloning process.OmpX gene product was expressed with E.coli expression system and the expressed rOmpX was extracted by Ni-NTA

关 键 词：SALMONELLA protective outer sequenced cloning immunized ISOLATES vaccines AFFINITY conservation 

分 类 号：R392[医药卫生—免疫学]