新鲜和冷冻猪卵子体外成熟培养对纤溶酶原激活物存在的影响  

Existence of plasminogen activators during in vitro maturation of fresh or frozen oocytes in the pig

作　　者：陈静波[1] 金文[1] 魏宁[1] 程汉[1] 尤明珍[1] 邓茂刚[1] 朴春槿 

机构地区：[1]江苏农牧科技职业学院,江苏泰州225300 [2]韩国江源大学,春川264006

出　　处：《中国兽医学报》2015年第6期1016-1021,共6页Chinese Journal of Veterinary Science

摘　　要：对体外成熟培养不同时间段猪的新鲜和冷冻的卵子中是否能够释放纤溶酶原激活物(PAs)的情况进行了研究。被卵丘细胞包裹的卵子取自于囊状卵泡,用NUSU-23培养液进行体外培养。OPS冷冻法冷冻卵子。在成熟培养液的卵丘细胞通过用细管不断地吸吐的方法去除。用SDS和酶谱法对猪的卵丘卵母细胞和裸卵里的纤溶酶原激活物进行密度测定。结果显示:在猪新鲜的卵丘卵母细胞中能够检测出Upa、tPA和tPA-PAI。体外培养24h后,在裸卵中也可以检测出uPA的活性;体外成熟培养48h后,在卵丘卵母细胞中能够检测出tPA和tPA-PAI,但在裸卵中没有检测出PAs。在所有冷冻组的试验中,没有检测出PAs活性。Whether the plasminogen activators(PAs)was produced by porcine fresh or frozenthawed cumulus-oocytes complexes(COCs)and cumulus cell free-oocytes was researched in this study.The Cumulus cell-enclosed oocytes were collected from antral follicles and cultured in NCSU-23 medium.COCs were frozen by open pulled straw(OPS)method.Cumulus cells were removed by repeated passge-through with a fine pipette in maturation medium.PA activities in porcine COCs and denuded-oocytes were quantified using SDS-PAGE,casein-agar zymography,and densitometry.The results suggested that uPA,tPA and tPA-PAI were produced by porcine COCs,but only uPA was detected in oocytes during maturation for 24 h.Only tPA,and tPA-PAI were produced by COCs cultured for 48 h,and no PAs were produced by denuded-oocytes cultured for48 h.At all of the frozen-thawed groups,no PAs were observed by COCs and oocytes.

关 键 词：纤溶酶原激活物 卵丘卵母细胞 OPS方法 体外成熟 

分 类 号：S814[农业科学—畜牧学]