健康水牛与患子宫内膜炎水牛子宫内细菌组成的比较分析  

Comparative analysis of uterine bacterial composition in healthy and endometritis buffaloes

作　　者：刘超逊[1,2] 梁辛[1] 杨承剑[1] 覃广胜[1] 韦升菊[1] 邹彩霞[1] 梁贤威[1] 杨炳壮[1] 

机构地区：[1]中国农业科学院广西水牛研究所,广西南宁530001 [2]广西大学动物科学技术学院,广西南宁530005

出　　处：《中国兽医学报》2015年第1期116-121,共6页Chinese Journal of Veterinary Science

基　　金：国家自然科学基金资助项目(31160470);广西水产畜牧兽医局科技研发项目(桂渔牧科1204916)

摘　　要：应用16SrDNA克隆文库的方法,分别建立健康和患子宫内膜炎水牛子宫内细菌的16SrDNA克隆文库,并通过序列分析和同源性比对确定细菌的系统发育学地位。结果显示,产后14d水牛子宫内的细菌主要属于梭杆菌门(Fusobacteria)、厚壁菌门(Firmicutes)、拟杆菌门(Bacteroidetes)、放线菌门(Actinobacteria)和变形菌门(Proteobacteria)。患子宫内膜炎组(M组)中有38.89%克隆的属于梭杆菌门(Fusobacteria),序列比对分析表明,这些序列和Fusobacterium necrophorum subsp有最高的的相似度(99%)。另外46.67%的克隆属于拟杆菌门(Bacteroidetes),其中Bacteroides heparinolyticus和Porphyromonas levii在这个门中所占比例最大,分别为55.95%和42.86%,属于厚壁菌门(Firmicutes)和变形菌门(Proteobacteri)的克隆较少,所占比例分别为7.22%和6.67%,但是这2个门中的细菌种类相对丰富。仅仅只有0.56%属于放线菌门(Actinobacteria)。健康组(H组)中,26.56%的克隆属于梭杆菌门(Fusobacteria),并且和Fusobacterium necrophorum subsp的相似度最高(99%),32.81%的克隆属于Firmicutes,这些克隆中的61.9%与Clostridium cadaveris的相似性最高(≧97%),35.94%的克隆属于Bacteroidetes,其中56.52%的序列与Bacteroides heparinolyticus相似性最高(99%)。克隆中属于Proteobacteria的比例较低(4.69%)。结果表明,Fusobacterium necrophorum subsp、Bacteroides heparinolyticus和Porphyromonas levii可能是引起水牛产后子宫内膜炎的主要致病菌。厚壁菌门细菌Clostridium cadaveris,拟杆菌门细菌Porphyromonas somerae、Bacteroides pyogenes可能有利于子宫内膜的恢复。The purpose of this study was to describe and compare the uterine bacterial composition in healthy and endometritic 14 days postpartum buffalo cows.Using culture-independent methods(clone library sequencing of broad-range 16 Sribosomal DNA PCR).Two 16 SrDNA clone libraries were constructed.Sequences were assigned to 5 major groups(Fusobacteria,Bacteroidetes,Proteobacteria,Firmicutes and Actinobacteria).Most of the clone sequences from the endometritic status libraries(38.89%)were affiliated with phylum Fusobacteria and all of these sequences had high identity(≧99%)with Fusobacterium necrophorum subsp.Several clone sequences were affiliated with the phylum Bacteroidetes(46.67%),sequences phylogenetically related to Bacteroides heparinolyticus(55.95%)and Porphyromonas levii(42.86%).Sequences related to Firmicutes(7.72%)and Proteobacteria(6.67%)were found in minor proportions,but bacteria in these two phylum are relatively diverse.Sequences affiliated with Actinobacteria(0.56%)were detected.In the clone libraries from the healthy status groups,the sequences were affiliated with Fusobacteria(26.56%)and all of these sequences had high identity(≧ 99%)with Fusobacterium necrophorum subsp.Additionally,32.81%of the sequences were affiliated with the phylum Firmicutes,with approximately 61.9% of the sequences showing high similarity(≧97%)with Clostridium cadaveris.Further,35.94% of the sequences were affiliated with the phylum Bacteroidetes,with approximately 56.52% of the sequences showing high similarity(99%)with Bacteroides heparinolyticus.In addition to these co-dominant phyla,sequences from Proteobacteria(4.69%)appear less frequently.The main pathogens of endometritis was Fusobacterium necrophorum subsp、Bacteroides heparinolyticus and Porphyromonas levii.Clostridium cadaveris、Porphyromonas somerae and Bacteroides pyogenes may be good for uterine health.

关 键 词：16S rDNA克隆文库 细菌多样性 水牛 子宫内膜炎 

分 类 号：S858.23[农业科学—临床兽医学]