在豚鼠体内筛选兔出血症病毒“通用型”T细胞表位  

Screening T cell epitopes of rabbit hemorrhagic disease virus in guinea pig

作　　者：金明兰[1,2,3] 霍晓伟[2] 南文龙[2] 鲁会军[2] 任静强[2] 刘春霞[2] 金宁一[2] 侯继波 

机构地区：[1]吉林建筑大学松辽流域水环境教育部重点实验室,吉林长春130118 [2]军事医学科学院军事兽医研究所,吉林长春130122 [3]国家兽医生物制品工程研究中心,江苏南京210114

出　　处：《中国兽医学报》2015年第2期213-217,共5页Chinese Journal of Veterinary Science

基　　金：江苏省博士后启动基金资助项目(0645)

摘　　要：为筛选兔出血症病毒T细胞表位,应用纯化的病毒与佐剂联合免疫6周龄豚鼠,三免后10 d采集血液和淋巴细胞,通过T淋巴细胞增殖试验(WST)、酶联免疫斑点(ELISPOT)、ELISA方法检测淋巴细胞增值、IFN-γ和IL-2变化,评价多肽的抗原性。结果显示,多肽P2、P7、P10、P16、P21和P22能刺激动物产生细胞免疫应答,淋巴细胞增殖、IFN-γ分泌和IL-2水平显著高于其他多肽和对照组。这表明,筛选的多肽P2、P7、P10、P16、P21和P22具有抗原特性,为多表位疫苗的构建和应用提供科学的依据。To screen whether derived peptides are authentic from 23 multi-peptides of RHDV,6-weeks-old guinea pig were immunized with purification RHDV and adjuvant respectively. The blood and lymphocyte were collected at 10 th day past the 3rd week re-immunization. Lymphocyte and IFN-γ change of immune guinea pig were detected by WST and ELISPOT respectively. IL-2 in serum of guinea pig was detected by ELISA. As a result,the P2,P7,P10,P16,P21 and P22 multi-peptide could significantly stimulate lymphocyte proliferation,IFN-γ and IL-2 secretion in immunized guinea pig. Animal immunity experiment revealed P2,P7,P10,P16,P21 and P22 multi-peptide could lead to specified reaction. The results showed that screening P2,P7,P10,P16,P21 and P22 peptides had immunogenicity,to provide a scientific basis for the construction and application of multi-peptides vaccines.

关 键 词：豚鼠 多肽 T细胞表位 兔出血症病毒 

分 类 号：S852.65[农业科学—基础兽医学]