制霉菌素对褐飞虱取食行为、营养及共生菌数量的影响  

Effects of nystatin on the feeding behavior,nutrition and yeast-like symbiont number of the brown planthopper,Nilaparvata lugens( St?l)

作　　者：张珏锋[1] 何月平[1,2] 陈建明[1] 

机构地区：[1]浙江省农业科学院植物保护与微生物研究所,浙江省植物有害生物防控重点实验室省部共建国家重点实验室培育基地,杭州310021 [2]华中农业大学植物科学技术学院,武汉430070

出　　处：《植物保护学报》2015年第2期264-270,共7页Journal of Plant Protection

基　　金：国家自然科学基金(30771411;31272040);浙江省自然科学基金(LY13C140008)

摘　　要：为明确制霉菌素对褐飞虱取食行为、营养和共生菌数量的影响,用150、300和600 U/m L制霉菌素处理的水稻饲养褐飞虱,利用刺探电位图、高效液相色谱和镜检法监测其取食行为及体内必需氨基酸含量和类酵母共生菌(yeast-like symbiotes,YLS)数量。结果表明,取食300、600 U/m L制霉菌素处理水稻的褐飞虱3龄若虫体内YLS数量显著低于未处理水稻(对照),而150 U/m L处理与对照无显著差异;5龄若虫体内YLS数量随制霉菌素浓度的增加而显著降低,但600 U/m L与300 U/m L处理间差异不显著。褐飞虱于韧皮部吸食波出现次数在对照中显著多于制霉菌素处理,不同浓度间无明显差异;制霉菌素对褐飞虱的吸食时间影响不明显,但对其体内必需氨基酸含量影响较大,600 U/m L处理下均显著低于对照和其余处理,300 U/m L处理下除丙氨酸、脯氨酸、蛋氨酸和酪氨酸外均显著低于对照,150 U/m L处理下也均显著低于对照。表明制霉菌素能显著降低褐飞虱体内YLS数量和各种必需氨基酸含量,但对其吸食时间的影响不明显。The effects of nystatin on the brown planthopper( BPH), Nilaparvata lugens, were determined by using electrical penetration graph( EPG), high performance liquid chromatography( HPLC) and microscopic examination. The BPHs were reared on the 0,150,300 or 600 U/m L concentrations of nystatin-treated rice plants,respectively. The results showed that the numbers of yeastlike symbiotes( YLS) in nystatin-treated 3rd instar BPH nymphs were lower than those of the control except the 150 U / m L treatment. The numbers of YLS in 5th instar BPH nymphs declined remarkably along with increasing nystatin concentration. But no significant difference was detected between 300 U /m L and 600 U / m L nystatin-treated populations. The frequency of N4-b( a parameter for determining the feeding behavior of BPH) was significantly higher in control population than that in all nystatin treated populations. However,no significant difference was detected among all nystatin treated concentrations.The nystatin had no effect on the feeding time of BPH,but it had a significant impact on the content ofessential amino acids. The contents of all essential amino acids of BPH in 600 U / m L nystatin treatment were significant lower than those of BPH in other treatments; the contents of most essential amino acids of BPH in 300 U / m L nystatin treatment and the contents of all essential amino acids of BPH in 150 U / m L nystatin treatment were also significantly lower than those of BPH in the control treatment. In conclusion,nystatin could significantly reduce the number of YLS and the content of essential amino acids in BPH,but no remarkable effects on feeding behavior.

关 键 词：褐飞虱 类酵母共生菌 制霉菌素 刺吸电位图 必需氨基酸 

分 类 号：S435.112.3[农业科学—农业昆虫与害虫防治]