重离子对人胃癌细胞DNA错配修复基因MSH2表达的影响  

Effects of Heavy Ion Irradiation on MSH2 Expression in Human Gastric Cancer Cell SGC7901

作　　者：缪国英[1,2,3,4,5] 张红[1,2,3,4] 卢启明[5] 李鸿岩[1,2,3,4] 狄翠霞[1,2,3,4] 郭逸潇[5] 

机构地区：[1]中国科学院近代物理研究所,兰州730000 [2]中国科学院重离子束辐射生物医学重点实验室,兰州730000 [3]甘肃省重离子束辐射医学应用基础重点实验室,兰州730000 [4]中国科学院大学,北京100049 [5]甘肃省人民医院,兰州730000

出　　处：《原子核物理评论》2015年第1期110-114,共5页Nuclear Physics Review

基　　金：国家重点基础研究发展计划(973计划)项目(2010CB834202);国家自然科学基金资助项目(10835011;10675151);甘肃省重大科技专项项目(0702NKDA045;0806RJYA020);中国科学院西部之光人才培养计划项目(XB106012);甘肃省科技支撑计划资助(1104FKCA155);甘肃省自然科学基金资助(096RJZA030)~~

摘　　要：采用高传能线密度(LET)重离子辐照人胃癌SGC7901细胞,应用流式细胞技术、蛋白质印迹法(Western blot)及反转录聚合酶链式反应(RT-PCR)观察重离子诱导人胃癌SGC7901细胞周期、凋亡和MSH2表达状况。结果表明:与对照组相比,SGC7901细胞在辐射后72 h G2/M期所占细胞比率(33.26±0.08)和凋亡率(24.16±0.64)均达到峰值,且呈时间依赖性增加;经重离子照射后,DNA错配修复基因MSH2 m RNA和蛋白表达水平在6 h最高。结果提示:重离子在体外诱导SGC7901细胞周期阻滞和凋亡,且具有显著的时间依赖性效应;重离子在一定剂量和时间下,诱导了SGC7901细胞MSH2基因表达。DNA错配修复基因MSH2可能参与了重离子辐照诱导胃癌细胞DNA损伤的修复应答。Human gastric cancer cell SGC7901 were irradiated with high linear energy transfer (LET) carbon ion. Apoptotic cells after irradiation were analyzed by flow cytometry and expression of MSH2 genes in the irradiated cells was detected by western blot and RT-PCR assay. Compared with the control group, we found that the number of G2/M (33.26 ± 0.08) or apoptosis (24.16 ± 0.64) of SGC7901 cells reached a maximum after irradiation at 72 h in a dose dependent manner. And heavy ion irradiation e?ciently up-regulated the expression of MSH2 gene at 4.0 Gy after being irradiated 6 h. These results imply that heavy ion beam could induce cell apoptosis and cell cycle arrest in time-dependent manners. Furthermore, expression of MSH2 genes activated by carbon ion irradiation suggests that DNA mismatch repair gene MSH2 might be involved in DNA repair pathways.

关 键 词：细胞凋亡 重离子束 MSH2 基因表达 

分 类 号：R735.2[医药卫生—肿瘤]