金纳米粒子的放射增敏效应研究  

Research on the Radiosensitizing Effect of Gold Nanoparticles

作　　者：刘玺[1,2,3] 刘岩[1,2,3] 陈卫强[1,2] 李强[1,2] 

机构地区：[1]中国科学院近代物理研究所,兰州730000 [2]中国科学院重离子束辐射生物医学重点实验室,兰州730000 [3]中国科学院大学,北京100049

出　　处：《原子核物理评论》2015年第2期230-235,共6页Nuclear Physics Review

基　　金：国家重点基础研究发展规划项目(973计划)(2010CB834203);国家自然科学基金委员会-中国科学院大科学装置联合基金资助项目(U1232207);国家自然科学基金资助项目(11205217);甘肃省杰出青年基金项目(1111RJDA010)~~

摘　　要：系统阐述了与金纳米粒子(GNPs)放射增敏效应相关实验的方法与结果、影响GNPs放射增敏的因素、GNPs放射增敏的细胞和动物实验表现及其相关机制。同时结合相关实验,分析和比较了15 nm柠檬酸钠包被的GNPs的放射增敏效应,发现GNPs在高LET的碳离子束和低LET的X射线辐照下对Hela细胞的杀伤力随其浓度的增加而增大;在50%的细胞存活率下,当GNPs的质量浓度为7.5μg/m L时,其X射线的剂量减少率和碳离子的相对生物学效应值(RBE)的增加率达到了最大,分别为65.3%和43.6%,同时GNPs共培养细胞24和48 h后,未出现细胞周期同步化的现象。This paper describes the methods and results of the previous experiments, the experimental phe-nomena of the cell and animal tests and the relative mechanisms on the radiosensitizing effect of GNPs. Together with our experiments, the radiosensitizing effects of 15 nm citrate-capped GNPs and related mechanisms are ana-lyzed and compared, finding that Hela cell killing of GNPs increase along with their concentration after exposure to high- and low-LET radiation such as carbon ions and X-rays. In addition, the percentages of dose reduction of the X-rays and RBE increment of the carbon ions reached their maximums 65.3% and 43.6%, respectively, at 50% survival level when Hela cells were pre-treated with 7.5 μg/mL GNPs. Moreover, Hela cells showed no cell-cycle synchronization after 24 and 48 h exposure to GNPs.

关 键 词：金纳米粒子 放射增敏效应 肿瘤治疗 

分 类 号：R730.55[医药卫生—肿瘤]