内质网应激对碳离子辐照宫颈癌HeLa细胞的影响  

Effect of Endoplasmic Reticulum Stress on the Response of HeLa Cells to Carbon Ion Radiation

作　　者：夏洁芳 王转子[1] 魏巍[1] 党秉荣[1] 李文建[1] 

机构地区：[1]中国科学院近代物理研究所,兰州730000 [2]兰州大学,兰州730000

出　　处：《原子核物理评论》2015年第2期236-241,共6页Nuclear Physics Review

基　　金：国家自然科学基金资助项目(11105189;11179002)~~

摘　　要：利用不同剂量的碳离子辐照二硫苏糖醇(2.5 mmol/L)预处理的HeLa细胞,探讨了内质网应激反应对碳离子辐照宫颈癌HeLa细胞的影响。实验发现:与单独辐照组相比,二硫苏糖醇联合碳离子辐照后细胞的存活率下降,而凋亡率增加;二硫苏糖醇联合碳离子辐照加重了碳离子辐照引起的细胞周期阻滞;且联合辐照组的自噬被明显激活。结果表明,持续的内质网应激可改变宫颈癌HeLa细胞对碳离子辐照反应,且二硫苏糖醇可能通过影响HeLa细胞的自噬性细胞死亡通路发挥作用。To investigate the effect of endoplasmic reticulum stress on HeLa cells to 12C6+ ion irradiation, HeLa cells were pretreated with 2.5 mmol/L dithiothreitol and irradiated by 12C6+ ions with different doses. The results showed that, compared with IR alone, dithiothreitol combined with carbon ion irradiation caused HeLa cell survival decreased, and the apoptosis increased. Moreover, dithiothreitol and carbon ion radiation combination treatment led to a significant increase of G2/M phase, and autophagy was activated obviously in combination treatment group. The results imply that continuous endoplasmic reticulum stress can change the response of HeLa cells to 12C6+ irradiation, and dithiothreitol may affect HeLa cells through the autophagy cell death pathway.

关 键 词：碳离子辐照 二硫苏糖醇 细胞凋亡 细胞周期 自噬 

分 类 号：R737.33[医药卫生—肿瘤]