茶皂素对种子发芽、根长及土壤酶活性的影响  

Effects of Tea Saponin on Seed Germination, Root Lengths and Soil Enzyme Activities

作　　者：侯俊杰[1] 李宁宁[1] 吕辉雄[1] 曾巧云[1] 吴启堂[1] 

机构地区：[1]华南农业大学资源环境学院,广州510642

出　　处：《农业环境科学学报》2015年第4期660-665,共6页Journal of Agro-Environment Science

基　　金：国家高技术研究发展计划(863计划)(2012AA101403);国家自然科学基金(41471215)

摘　　要：选取玉米、绿豆和菜心作为研究对象,调查茶皂素对3种作物种子发芽及根伸长的影响情况,同时研究茶皂素对土壤过氧化氢酶活性和多酚氧化酶活性的影响。结果表明:试验浓度范围内,茶皂素溶液对玉米、绿豆和菜心种子的发芽率及根伸长均具有显著的抑制作用(绿豆种子发芽率除外),0.2%处理对3种种子根伸长的抑制率达33.1%以上,其根长显著低于对照处理,对玉米和菜心种子发芽率的抑制率达10%;茶皂素对种子发芽率及根长抑制作用的强弱与种子类型有关,整体来说,茶皂素对3种种子发芽率的抑制作用由强到弱依次为玉米>菜心>绿豆,对3种种子根伸长的抑制作用由强到弱依次为菜心>玉米>绿豆。茶皂素对土壤过氧化氢酶活性具有显著的抑制作用,而对多酚氧化酶活性则具有显著的促进作用。Tea saponin, as a biosurfactant, has great potential to be applied to the environmental remediation. However, it has certain biological toxicity to soil ecosystem. In this study, seeds of Chinese flowering cabbage(Brassica parachinensis), mung bean(Vigna radiata L.)and maize(Zea mays L.)were selected to examine the influences of tea saponin added to soil on seed germination rates, root elongations, and the activities of soil catalase and polyphenol oxidase. Results showed that the seed germination rates(excluding mung bean)and root lengths of three plant species were significantly inhibited by tea saponin at the experimental concentrations. The inhibitory effects aggravated with increasing concentrations of tea saponin. When tea saponin concentration was 0.2%, the root lengths significantly decreased more than 33.1%and the germination rates reduced more than 10%, compared with the control(without tea saponin). The inhibition of tea saponin on germination rates and root lengths were dependent on the plant types. The germination rates decreased in order of maize > Chinese flowering cabbage > mung bean, while root lengths decreased in order of Chinese flowering cabbage > maize > mung bean. With increasing concentrations of tea saponin, catalase activity in soil decreased significantly, while soil polyphenol oxidase activity increased significantly.

关 键 词：茶皂素 种子发芽 根长 酶活性 生态效应 

分 类 号：X171.5[环境科学与工程—环境科学]