耐铜细菌及其菌群对纳米CuO的淋滤作用研究  

Leaching of CuO Nanoparticles with Copper Tolerant Bacteria and Their Communities

作　　者：昝树婷[1] 周刚[1] 李静[1] 苏楠楠[1] 邵宗圆 杨如意[1] 

机构地区：[1]安徽师范大学环境科学与工程学院,安徽芜湖241003

出　　处：《农业环境科学学报》2015年第6期1082-1089,共8页Journal of Agro-Environment Science

基　　金：国家自然科学基金(41001368);安徽高校省级自然科学研究重点项目(KJ2009A026)

摘　　要：通过PCR-DGGE和测序等方法分析了铜累积性植物茵陈蒿(Artemisia capillaris Thunb.)根际的细菌群落,并研究了耐铜细菌对纳米Cu O(Cu O NPs)的淋滤作用,试图提高Cu O NPs的生物有效性,以加速修复进程。结果表明,茵陈蒿根际细菌多样性很低,主要由气球菌属、寡养单胞菌属和微球菌属组成,其中气球菌属为优势属。低浓度Cu O NPs处理对细菌S31的生长有一定促进作用,但400 mg·L-1的Cu O NPs则会对其产生明显抑制;不同浓度下,细菌S31对Cu O NPs的淋滤作用有明显差异;驯化不仅明显改善了细菌的生长状况,还进一步增强了Cu O NPs的淋滤。通过微生物提高人工纳米材料生物有效性的方法能否实际用于环境修复还有待进一步的试验进行验证。Behavior, environmental fate and biotoxicity of manufactured nano materials(MNMs)have received extensive attention in recent years. However, it is unclear whether MNMs-contaminated soils could be remediated by combined plant and rhizospheric microbes. In this study, bacterial community structure associated with copper(Cu) accumulating plant Artemisia capillaris Thunb. was investigated using PCR-DGGE and sequencing techniques. Subsequently, Cu tolerant bacteria were screened using selective culture medium containing 500mg·L-1Cu SO4. The effect of Cu tolerant bacteria and bacterial communities on leaching of nano Cu O(Cu O NPs)was then studied. Results showed that bacterial community in the rhizosphere had very low diversity, and mainly consisted of genus of Aerococcus, Stenotrophomonas and Micrococcus, with Aerococcus being the dominant genus. The growth of bacterium S31 was enhanced by low Cu O NPs, but suppressed by 400 mg·L-1Cu O NPs. The Cu tolerant bacterium S31 showed a concentration dependent enhancement on leaching of Cu O NPs. Acclimation significantly improved the growth of bacterium S31 and increased the leaching of Cu O NPs. This study indicates the potential to remediate MNMs-contaminated soil by combined plant and microbes.

关 键 词：重金属 驯化 淋滤 

分 类 号：X172[环境科学与工程—环境科学]