温控气体损伤建立兔动脉粥样硬化易损斑块模型  

A Rabbit Model of Vulnerable Atherosclerotic Plaque Induced by Temperature-controlling Gas

作　　者：路怀志 杜大勇[1] 柳杨[1] 李沫言[1] 薛峰[1] 杨升华[1] 张雁斌[1,2] 李运田[1,2] 

机构地区：[1]中国人民解放军第305医院心脏中心,北京市100017 [2]南方医科大学第二临床医学院,广东省广州市510515

出　　处：《中国动脉硬化杂志》2015年第2期132-136,共5页Chinese Journal of Arteriosclerosis

基　　金：全军"十一五"医药卫生科研基金项目(06G145)

摘　　要：目的利用持续恒定低温气体损伤血管内皮,结合高脂饮食,建立兔颈动脉粥样硬化易损斑块动物模型。方法新西兰雄性白兔24只,3月龄,随机分为空白对照组、假手术组和温控气体损伤组,每组8只。高脂饮食喂养1周后对温控气体损伤组利用低温气体给予血管内膜损伤处理,假手术组单纯给予有创手术操作,术后两组均继续给予高脂饮食12周;空白对照组给予持续普通饮食。术后13周处死动物,取血管行HE、Masson、弹力纤维、油红O染色,观察血管形态,并做小鼠抗兔巨噬细胞抗体11免疫组织化学染色。结果温控气体损伤组可见薄的纤维帽、大脂质核心,内膜有巨噬细胞及泡沫细胞浸润,部分内皮脱落,斑块出现裂隙,局部小血栓形成,形成典型的易损斑块;假手术组多表现为内膜增生,部分存在小斑块,内皮下见泡沫细胞浸润,符合脂纹期改变,为初级斑块;空白对照组未见斑块形成。结论利用恒定低温气体损伤兔颈动脉内膜结合高脂饮食可建立典型的易损斑块模型,且方便易行。Aim To develop a rabbit model of vulnerable atherosclerotic plaque by cryogenic gas-induced endothelial injury combined with high-fat diet. Methods 24 healthy male New Zealand rabbits( 3 months) were randomly divided into three groups: control blank group( group A),sham-operated group( group B) and temperature control gas injury group( group C) with 8 in each group. After one week high-fat diet,the rabbits in group C were underwent carotid artery intima injury using cryogenic gas. The rabbits in group B suffered surgical operations without cryogenic gas. After the operation,the rabbits in group B and group C were kept feeding on high-fat diet for 12 weeks,whereas the ones in group A were kept on normal diet. All rabbits were killed after 13 weeks. Then paraffin-embedded and frozen sections were used for HE staining,Masson trichrome staining and elastic fiber staining and oil red O staining to analyze the changes of vascular morphology. Additionally,rat anti-rabbit macrophage antibody 11( RAM11) monoclonal antibody was applied for animal immunohistochemistry staining. Results The rabbit carotid atherosclerosis established in our study was in line with the progress characteristics of vulnerable plaque,involving thin fibrous cap,large lipid core and macrophages and foam cells in the intima as well as endothelial cells shedding or local small scattered thrombosis. The rabbit carotid in sham-operated group showed intimal hyperplasia and partly showed small primary plaques infiltrated by macrophages,which was in line with the change of fatty streaks phase. There is no plaque visible in the control blank group. Conclusion It is viable and easy to develop a rabbit model of typical vulnerable atherosclerotic plaque by injuring carotid intima with temperature control gas combined with high-fat diet.

关 键 词：易损斑块 动物模型 内皮损伤 恒温气体 

分 类 号：R-332[医药卫生] R543.5