基于线粒体COI基因序列的中国鲷科鱼类系统进化关系  

PHYLOGENETIC RELATIONSHIP IN FAMILY SPARIDAE OF CHINA IN MITOCHONDRIAL COI GENE SEQUENCE

作　　者：陈咏霞[1] 吴仁协[2] 梁娜[1] 刘静[3] 

机构地区：[1]河北大学生命科学学院,保定071002 [2]广东海洋大学水产学院,湛江524088 [3]中国科学院海洋研究所海洋生物分类与系统演化研究室,青岛266071

出　　处：《海洋与湖沼》2015年第3期611-619,共9页Oceanologia Et Limnologia Sinica

基　　金：国家自然科学基金资助项目;31172053号;41276166号;41006084号;31372166号;广东省高等学校优秀青年教师培养计划资助项目;Yq2013093号

摘　　要：采用PCR特异性扩增了采集于中国沿海的鲷科5属11种鱼类的线粒体COI基因序列,并结合Gen Bank已有的同源序列,对中国鲷科6属12种鱼类的分子系统进化关系进行研究。结果表明,中国鲷科6属12种鱼类的COI基因序列的平均碱基组成为T 30.4%、C 27.7%、A 23.5%、G 18.4%;属间Kimura双参数平均遗传距离为9.5%—19.7%,种间平均遗传距离为2.2%—21.8%,其中四长棘鲷属、赤鲷属和犁齿鲷属间的遗传距离(8.2%—13.8%)接近于棘鲷属多数种间遗传距离(6.5%—12.2%);分子系统树显示中国鲷科鱼类分为两大类群,类群I包括四长棘鲷属、犁齿鲷属、赤鲷属、牙鲷属等红体色种类,类群Ⅱ含平鲷属、棘鲷属等银灰体色种类,其中棘鲷属的冲绳棘鲷和黄鳍棘鲷先聚为姐妹群,澳洲棘鲷、黑棘鲷、灰鳍棘鲷和台湾棘鲷聚为另一支。本文结合已有研究结果得出:中国鲷科鱼类属间是先按体色、后按牙齿分化的,四长棘鲷属、犁齿鲷属和赤鲷属有相当密切的亲缘关系;中国棘鲷属是一个有共同祖先的单系群,属内存在两个平行进化的分支,两分支的种间关系具有明显的分化。The mitochondrial COI gene sequences of 11 species pertaining to 5 genera of Sparidae(Perciformes) collected from Chinese coastal waters, were amplified using PCR techniques. The homologous sequences of other Chinese species of Sparidae from Gen Bank were compared in this study. The results indicate that the average contents of T, C, A and G were 22.2%, 24.5%, 30.0%, and 23.3%, respectively for the sparid fishes analyzed in this study(6 genera and 12 species). The genetic distances among genera and species ranged 9.5%—19.7%, and 2.2%—21.8%, respectively in Kimura-2-parameter model. The genetic distances among Argyrops, Pagrus, and Evynnis were 8.2%—13.8%, very close to those between two species for Acanthopagrus. Molecular phylogenic analysis supported strongly that two distinct clades in Family Sparidae. One clade includes red color fishes, Dentex, Argyrops, Evynnis, and Pagrus. The other clade includes silver gray color fishes, Acanthopagrus and Rhabdosargus. Within Genera Acanthopagrus, A. chinshira and A. latus formed firstly a sister clade, while A. schlegelii, A. berda, A. taiwanensis, and A. australis were grouped into another. Based on the results in this study and preview studies, we believe that: 1) Chinese species of Sparidae diverged first by body color, and then by dentition; 2) Argyrops, Pagrus, and Evynnis are closely related species; and 3) the Chinese species of Acanthopagrus joined into a monophyletic group. The monophyletic group had been divided into two co-evolutionary clades with clear species-level differences. However, the position of A. australis remains uncertain within Acanthopagrus.

关 键 词：鲷科 COI基因 系统进化 

分 类 号：S917.4[农业科学—水产科学]