莲种质花粉形态特征研究  

Studies on Pollen Morphology of Nelumbo Germplasm Resources

作　　者：李佐[1,2] 赵凯歌[1] 赵玫[1] 陈龙清[1] 

机构地区：[1]华中农业大学园艺林学学院,园艺植物生物学教育部重点实验室,武汉430070 [2]广东省农业科学院环境园艺研究所,广州510640

出　　处：《园艺学报》2015年第1期75-85,共11页Acta Horticulturae Sinica

基　　金：国家自然科学基金项目(31272197)

摘　　要：利用扫描电镜对不同来源的31份莲种质的花粉形态进行了观察。研究结果表明:花粉外部形态均呈近球形,赤道面观均为椭圆形,极面观大部分呈三裂圆形,少量种质有近圆形。所有莲种质除都具3条萌发沟外,在5个种质中还首次发现具有散沟的花粉(六沟花粉)。不同种质间花粉形态特征具有差异,尤其是萌发器官和外壁纹饰细部特征的差别,体现了不同莲种质的遗传多样性和遗传分化,可为种质间亲缘关系及系统发育研究提供有价值的依据。Pollens gains from 31 Nelumbo germplasm resources were examined using scanning electron microscopy(SEM). The results showed that all pollen grains investigated are sub-spheroidal in shape and elliptical in equatorial view. However,most of the pollen grains are 3-lobed-round type in polar view,and the others are sub-orbicular. The pollen grains of all the accessions exclusively are 3-colpate type,and five accessions are additional 6-pantocolpate type. The obvious differences of pollen morphology of those 31 Nelumbo germplasm,especially in the characters of colpus and lirae demonstrated the genetic diversity and divergence,which provided valuable tools to analyze the phylogenetic relationship among the Nelumbo accessions.

关 键 词：莲 花粉形态 种质资源 遗传多样性 扫描电镜 

分 类 号：S682.32[农业科学—观赏园艺]