一株施氏假单胞菌Pseudomonas stutzeriDN-LWX19的脱氮性能  

Denitrification performance of Pseudomonas stutzeri DN-LWX19

作　　者：周晓黎[1,2] 孙迎雪[1] 沈丹丹[1] 杨哲[1] 田媛[1] 

机构地区：[1]北京工商大学环境科学与工程系,北京100048 [2]清华大学国家环境保护环境微生物利用与安全控制重点实验室,北京100084

出　　处：《环境工程学报》2015年第1期247-252,共6页Chinese Journal of Environmental Engineering

基　　金：北京市教委科技面上项目(KM201210011004);国家环境保护环境微生物利用与安全控制重点实验室开放基金资助项目(SMARC2013D002)

摘　　要：从缺氧生物滤池筛选纯化得到一株具有高效反硝化能力的异养菌DN-LWX19,通过形态观察、生理生化特性及16S r DNA同源性分析,确定该菌株为施氏假单胞菌(Pseudomonas stutzeri)。脱氮性能研究结果表明,菌株DN-LWX19在NO-3-N初始浓度为120.43 mg/L的试管培养基中,60 h对NO-3-N去除率达90%,NO-2-N积累浓度仅0.05 mg/L。以添加适量乙酸钠的实际污水处理二级出水为液体培养基,研究DN-LWX19在初始NO-3-N浓度约为30.00 mg/L时的脱氮效果,当碳氮比(COD/NO-3-N)为5∶1时,菌株DN-LWX19在32 h对NO-3-N去除率为100%,但是有NO-2-N的积累(7.00 mg/L),且至72 h无明显变化;当碳氮比为9∶1时,菌株DN-LWX19在32h对NO-3-N的去除率为100%,且无NO-2-N的积累。A heterotrophic bacteria DN-LWX19,with a highly efficient denitrification capacity,was isolated from a lab-scaled anoxic biofilter. DN-LWX19 was identified as Pseudomonas stutzeri according to its morphological,physiological and biochemical properties and phylogenetic analysis by the 16 S r DNA sequence. The results showed that,the removal efficiency of NO-3-N was 90% and the accumulation of NO-2-N was only 0. 05 mg / L after the 60 h denitrification in the vitro medium under the influent NO-3-N of 120. 43 mg / L. When the DN-LWX19 was cultivated in the synthetic wastewater secondary treated effluent adding the appropriate amount of CH3 COONa with NO-3-N concentration of 30. 00 mg / L,the removal efficiency of NO-3-N reached 100% under the 32 h denitrification at initial C / N( COD / NO-3-N) ratio of 5 ∶ 1,but the accumulation of NO-2-N was 7. 00 mg / L and without any change until 72 h cultivation. Moreover,the removal efficiency of NO-3-N reached 100% without any accumulation of NO-2-N under the 32 h denitrification at initial C / N ratio of 9 ∶ 1.

关 键 词：反硝化 脱氮 碳氮比 16SrDNA 施氏假单胞菌 

分 类 号：X172[环境科学与工程—环境科学]