异养硝化-好氧反硝化菌ADN-42的脱氮特性  

Nitrogen-removal characteristic of heterotrophic nitrification and aerobic denitrification bacterium ADN-42

作　　者：刘天琪[1,2] 金若菲[1,2] 周集体[1,2] 黄剑宇[1,2] 

机构地区：[1]大连理工大学环境学院,大连116024 [2]大连理工大学工业生态与环境工程教育部重点实验室,大连116024

出　　处：《环境工程学报》2015年第2期989-996,共8页Chinese Journal of Environmental Engineering

基　　金："十二五"国家科技支撑计划项目(2012BAD15B05);中央高校基本科研业务费专项(DUT14LAB09)

摘　　要：从大连海域典型繁茂膜海绵(Hymeniacidon perleve)中筛选出1株耐盐异养硝化-好氧反硝化菌,通过形态观察、生理生化实验和16S rRNA基因序列分析,确定其为假单胞菌属(Pseudomonas),命名为ADN-42。其异养硝化-好氧反硝化条件为氯化铵为氮源,柠檬酸三钠为碳源,温度30℃,C/N值为12,摇床转速150 r/min,NH+4-N初始浓度约300 mg/L,盐度为40 g/L Na Cl,在此条件下菌株84 h时NH+4-N去除率为75.4%,无硝态氮产生,亚硝态氮最大积累量为8.3 mg/L;将菌株投加到NH+4-N和NO-2-N混合体系中,混合系统比仅以NH+4-N为氮源的体系的NH+4-N去除速率提高了12.7%;研究结果表明Pseudomonas sp.ADN-42可能是一株有着良好应用前景的高效耐盐异养硝化-好氧反硝化菌。A novel heterotrophic ammonium(NH+4-N) removal bacterial strain,designated as ADN-42,was isolated from Hymeniacidon perleve.Strain ADN-42 was identified as Pseudomonas sp.by morphological and16 S rRNA sequence analyses.The ammonia nitrogen removal rate was 75.4% without NO-3accumulation and with a maximum NO-2accumulation of 8.3 mg/L in 84 h under the following conditions:carbon source,sodium citrate;nitrogen source,ammonium chloride;C/N of 12;temperature of 30℃;shaking speed of 150 r/min;p H of 7.0;initial ammonium nitrogen of 300 mg/L;and Na Cl of 40 g/L.When the strain was added to the system containing both NH+4-N and NO-2-N,the NH+4-N removal rate was 12.7% higher than that of the system using only NH+4-N as nitrogen source.The results showed that Pseudomonas sp.ADN-42 could be an efficient heterotrophic nitrification-aerobic denitrification bacterial strain in hypersaline environment.

关 键 词：异养硝化 好氧反硝化 PSEUDOMONAS sp.ADN-42 

分 类 号：X172[环境科学与工程—环境科学]