Pseudomonas sp.fz胞外分离物降解四溴双酚A  

Degradation of tetrabromobisphenol-A by extracellular isolates of Pseudomonas sp. fz

作　　者：刘沙沙[1,2] 王竞[1,2] 吕红[1,2] 柳广飞[1,2] 范真真[1,2] 周集体[1,2] 

机构地区：[1]大连理工大学环境学院,大连116024 [2]大连理工大学工业生态与环境工程教育部重点实验室,大连116024

出　　处：《环境工程学报》2015年第4期1631-1638,共8页Chinese Journal of Environmental Engineering

基　　金：国家自然科学基金资助项目(21077021);国家"水体污染控制与治理"科技重大专项(2012ZX07202-006)

摘　　要：四溴双酚A(TBBPA)是全球消耗量最大的溴系阻燃剂。通过活性物质的定位、分离和TBBPA产物的分析等步骤,对Pseudomonas sp.fz胞外分离物降解TBBPA进行了研究。结果表明,存在于胞外的活性物质通过异丙基断裂和脱溴两条途径降解TBBPA。通过超滤,Sephadex G-10分离纯化得到了具有降解活性的小分子物质,分子量约为376~456 Da,初步鉴定为短肽类物质。这种小分子物质具有很好的热稳定性(30~80℃),在弱酸性条件下(2.0~5.0)活性较高,其活性还受金属离子、氧气和抑制剂(Na N3)的影响。此外,部分纯化的小分子物质在一定条件下能够产生羟基自由基(·OH)。Tetrabromobisphenol-A( TBBPA) is the most widely used brominated flame retardant in the world. The degradation of TBBPA by extracellular isolates of Pseudomonas sp. fz was studied due to the location and separation of active components,the analysis of TBBPA metabolites. The results demonstrated that the extracellular compounds were able to degrade TBBPA via two pathways( isopropyl rupture and debromination). The low molecular mass components( LMMCs) with degrading activity separated and purified by ultra filtration and Sephadex G-10 were identified to be peptide preliminarily. Its molecular mass ranged from 376 Da to 456 Da. The LMMCs showed thermostability over a wide range of temperatures( 30 ~ 80℃),and high activity in the acidic p H range( 2. 0 ~ 5. 0). The activity of the LMMCs was also affected by mental salts,inhibitor( Na N3) and oxygen. In addition,hydroxyl radical( ·OH) was produced by partial purified LMMCs.

关 键 词：PSEUDOMONAS sp.fz 四溴双酚A 胞外小分子 非酶降解过程 

分 类 号：X172[环境科学与工程—环境科学] X505